Endometriosis is a common gynecologic disease involving endometrial tissue located outside of the uterine cavity. It causes debilitating pain and frustrating infertility in women with this disease. Despite extensive nvestigation, no study has shown a specific gene involved in endometriosis, but a genetic predisposition is likely. To define the molecular profile of women with endometriosis, we have recruited 84 endometriosis cases in the Houston area and 47 control patients. Genomic DMA is being analyzed by comparative genomic hybridization using the Agilent platform, mRNA levels are analyzed with Illumina microarrays, and microRNAs by the Illumina/Solexa sequencing platform. The overall hypothesis for this project is that regulatory defects in microRNAs play a central early role in organizing the molecular changes involved in endometriosis and, in doing so, determine important aspects of its clinical behavior and response to therapy.
The specific aims of the project involve identifying microRNA regions in genomic DNA associated with endometriosis, profiling microRNA signatures of normal endometrium and endometriotic tissues, validating microRNA:mRNA targets, and creating in vitro models for studying the role of candidate microRNAs in uterine function and dysfunction. Our proposed research will allow us to develop cost-effective, highthroughput strategies for classifying individual patients with endometriosis at a molecular (microRNA, mRNA, and genomic) level with the long-term goal of developing patient-specific treatment protocols.

Public Health Relevance

Endometriosis is a devastating disease to women, involving pain, decreased quality of life, and infertility. Currently, accurate diagnosis requires surgery and treatment protocols are grossly ineffective long term. The goals of our studies are to develop a molecular signature for each patient with endometriosis that can be used diagnostically to assess prognosis and develop novel therapeutic interventions.

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Specialized Center--Cooperative Agreements (U54)
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Special Emphasis Panel (ZHD1-DSR-L)
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Baylor College of Medicine
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Lloyd, Richard E (2016) Enterovirus Control of Translation and RNA Granule Stress Responses. Viruses 8:93
Ren, Yi A; Liu, Zhilin; Mullany, Lisa K et al. (2016) Growth Arrest Specific-1 (GAS1) Is a C/EBP Target Gene That Functions in Ovulation and Corpus Luteum Formation in Mice. Biol Reprod 94:44
Toneff, M J; Sreekumar, A; Tinnirello, A et al. (2016) The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states. BMC Biol 14:47
Trial, JoAnn; Cieslik, Katarzyna A; Entman, Mark L (2016) Phosphocholine-containing ligands direct CRP induction of M2 macrophage polarization independent of T cell polarization: Implication for chronic inflammatory states. Immun Inflamm Dis 4:274-88
Giudice, Jimena; Loehr, James A; Rodney, George G et al. (2016) Alternative Splicing of Four Trafficking Genes Regulates Myofiber Structure and Skeletal Muscle Physiology. Cell Rep 17:1923-1933
Matatall, Katie A; Jeong, Mira; Chen, Siyi et al. (2016) Chronic Infection Depletes Hematopoietic Stem Cells through Stress-Induced Terminal Differentiation. Cell Rep 17:2584-2595
McConnell, Kellie I; Shamsudeen, Sabeel; Meraz, Ismail M et al. (2016) Reduced Cationic Nanoparticle Cytotoxicity Based on Serum Masking of Surface Potential. J Biomed Nanotechnol 12:154-64
Han, Sang Jun; Begum, Khurshida; Foulds, Charles E et al. (2016) The Dual Estrogen Receptor α Inhibitory Effects of the Tissue-Selective Estrogen Complex for Endometrial and Breast Safety. Mol Pharmacol 89:14-26
Huq, Redwan; Samuel, Errol L G; Sikkema, William K A et al. (2016) Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation. Sci Rep 6:33808
Szafran, Adam T; Mancini, Maureen G; Nickerson, Jeffrey A et al. (2016) Use of HCA in subproteome-immunization and screening of hybridoma supernatants to define distinct antibody binding patterns. Methods 96:75-84

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