A. Objective The objective of Core C is to develop and disseminate experimental and computational technologies that are critical for studying the molecular genetics and genomics of small biological samples, as exemplified by germ cell or pre-implantation embryo samples. We will take two main directions to accomplish this objective:
Aim 1. Develop experimental protocols to apply massively parallel RNA-sequencing (RNA-Seq) to discover novel, splice and other 3'UTR variants and their differential roles in development, with a special emphasis on understanding the post-transcriptional regulatory function of 3'UTR variants.
Aim 2. Host and disseminate validated experimental protocols, computational tools and annotated RNA-Seq data sets to make next-generation sequencing, single-cell analysis, and microfluidics techniques accessible to the Reproductive Biology, SCCPRIR, and broader scientific communities.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD068158-03
Application #
8446124
Study Section
Special Emphasis Panel (ZHD1-DSR-L)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
3
Fiscal Year
2013
Total Cost
$185,959
Indirect Cost
$74,147
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Durruthy-Durruthy, Jens; Sebastiano, Vittorio; Wossidlo, Mark et al. (2016) The primate-specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming. Nat Genet 48:44-52
Ramathal, Cyril; Angulo, Benjamin; Sukhwani, Meena et al. (2015) DDX3Y gene rescue of a Y chromosome AZFa deletion restores germ cell formation and transcriptional programs. Sci Rep 5:15041
Baker, Catherine Craig; Gim, Byung Soo; Fuller, Margaret T (2015) Cell type-specific translational repression of Cyclin B during meiosis in males. Development 142:3394-402
Briggs, Sharon F; Dominguez, Antonia A; Chavez, Shawn L et al. (2015) Single-Cell XIST Expression in Human Preimplantation Embryos and Newly Reprogrammed Female Induced Pluripotent Stem Cells. Stem Cells 33:1771-81
Cheng, Yuan; Feng, Yi; Jansson, Lina et al. (2015) Actin polymerization-enhancing drugs promote ovarian follicle growth mediated by the Hippo signaling effector YAP. FASEB J 29:2423-30
Cheng, Yuan; Kim, Jaehong; Li, Xiao Xiao et al. (2015) Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators. PLoS One 10:e0117769
Hsueh, Aaron J W; Kawamura, Kazuhiro; Cheng, Yuan et al. (2015) Intraovarian control of early folliculogenesis. Endocr Rev 36:1-24
Briggs, Sharon F; Reijo Pera, Renee A (2014) X chromosome inactivation: recent advances and a look forward. Curr Opin Genet Dev 28:78-82
Durruthy-Durruthy, Jens; Briggs, Sharon F; Awe, Jason et al. (2014) Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. PLoS One 9:e94231
Durruthy Durruthy, Jens; Ramathal, Cyril; Sukhwani, Meena et al. (2014) Fate of induced pluripotent stem cells following transplantation to murine seminiferous tubules. Hum Mol Genet 23:3071-84

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