A. Objective The objective of Core C is to develop and disseminate experimental and computational technologies that are critical for studying the molecular genetics and genomics of small biological samples, as exemplified by germ cell or pre-implantation embryo samples. We will take two main directions to accomplish this objective:
Aim 1. Develop experimental protocols to apply massively parallel RNA-sequencing (RNA-Seq) to discover novel, splice and other 3'UTR variants and their differential roles in development, with a special emphasis on understanding the post-transcriptional regulatory function of 3'UTR variants.
Aim 2. Host and disseminate validated experimental protocols, computational tools and annotated RNA-Seq data sets to make next-generation sequencing, single-cell analysis, and microfluidics techniques accessible to the Reproductive Biology, SCCPRIR, and broader scientific communities.
|Durruthy Durruthy, Jens; Ramathal, Cyril; Sukhwani, Meena et al. (2014) Fate of induced pluripotent stem cells following transplantation to murine seminiferous tubules. Hum Mol Genet 23:3071-84|
|Dominguez, Antonia A; Chiang, H Rosaria; Sukhwani, Meena et al. (2014) Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. Sci Rep 4:6432|
|Ramathal, Cyril; Durruthy-Durruthy, Jens; Sukhwani, Meena et al. (2014) Fate of iPSCs derived from azoospermic and fertile men following xenotransplantation to murine seminiferous tubules. Cell Rep 7:1284-97|
|Durruthy-Durruthy, Jens; Briggs, Sharon F; Awe, Jason et al. (2014) Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. PLoS One 9:e94231|
|Reddy, Pradeep; Deguchi, Masashi; Cheng, Yuan et al. (2013) Actin cytoskeleton regulates Hippo signaling. PLoS One 8:e73763|
|Pera, Renee A Reijo (2013) Status of human germ cell differentiation from pluripotent stem cells. Reprod Fertil Dev 25:396-404|
|Medrano, Jose V; Pera, Renee A Reijo; Simon, Carlos (2013) Germ cell differentiation from pluripotent cells. Semin Reprod Med 31:14-23|
|Kawamura, Kazuhiro; Cheng, Yuan; Suzuki, Nao et al. (2013) Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment. Proc Natl Acad Sci U S A 110:17474-9|
|Hsueh, Aaron J; Rauch, Rami (2012) Ovarian Kaleidoscope database: ten years and beyond. Biol Reprod 86:192|
|Cheng, Yuan; Kawamura, Kazuhiro; Deguchi, Masashi et al. (2012) Intraovarian thrombin and activated protein C signaling system regulates steroidogenesis during the periovulatory period. Mol Endocrinol 26:331-40|
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