(same as original) This is a proposal to create a production center capable of implementing a pipeline that includes acquisition and evaluation of human transcription factor immunogens, production and selection of monospecific Monoclonal antibodies (mMAbs), and biochemical characterization and validation of these protein capture (affinity) reagents. We emphasize the advantages of high throughput and scalability to be achieved within our production effort, and propose the development of process improvements, thereby improving cost and quality over the duration of the award. We will maintain a degree of scientific flexibility to develop approaches to producing affinity reagents to less tractable transcription factor protein targets as they are identified in the course of production. Finally we propose a viable distribution plan consistent with NIH policies, utilizing the existing Developmental Hybridoma Monoclonal Bank, which ensures broad availability and wide accessibility for future use of the reagents with minimal constraints, consistent with achieving the goals of this funding initiative.

Public Health Relevance

(same as original) A consistent set of renewable protein Capture Reagents will facilitate NIH research in all areas relevant to human health as a basic tool used by virtually all NIH funded scientists. Monoclonal antibodies will also provide the diagnostic tools and therapeutic medicines of the future.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HG006434-03
Application #
8534873
Study Section
Special Emphasis Panel (ZRG1-BST-M (50))
Program Officer
Gatlin, Christine L
Project Start
2011-09-26
Project End
2016-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
3
Fiscal Year
2013
Total Cost
$1,777,257
Indirect Cost
$189,376
Name
Johns Hopkins University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Fan, Baochang; Lu, Kuan-Yi; Reymond Sutandy, F X et al. (2014) A human proteome microarray identifies that the heterogeneous nuclear ribonucleoprotein K (hnRNP K) recognizes the 5' terminal sequence of the hepatitis C virus RNA. Mol Cell Proteomics 13:84-92
Liu, S; Zhu, J H; He, L P et al. (2014) Label-free, real-time detection of the dynamic processes of protein degradation using oblique-incidence reflectivity difference method. Appl Phys Lett 104:163701
Hu, Jianfei; Rho, Hee-Sool; Newman, Robert H et al. (2014) PhosphoNetworks: a database for human phosphorylation networks. Bioinformatics 30:141-2
Hu, Shaohui; Feng, Yingzhu; Henson, Brandon et al. (2013) VirD: a virion display array for profiling functional membrane proteins. Anal Chem 85:8046-54
Sutandy, F X Reymond; Qian, Jiang; Chen, Chien-Sheng et al. (2013) Overview of protein microarrays. Curr Protoc Protein Sci Chapter 27:Unit 27.1
Uzoma, Ijeoma; Zhu, Heng (2013) Interactome mapping: using protein microarray technology to reconstruct diverse protein networks. Genomics Proteomics Bioinformatics 11:18-28