The Human and Mouse Cell Isolation and Phenotyping Core (Core C) will provide services for all four research projects in the University of Utah Molecular Medicine Translational Research Center in Thrombosis (U2M2-TRCT) program. Core C is centrally located and immediately available to all U2M2-TRCT investigators. The core will isolate human and mouse megakaryocytes and platelets for U2M2-TRCT investigators, perform standard in vitro and in vivo platelet function assays, assist with generation, breeding, and maintenance of genetically manipulated mice proposed in projects 1 and 2, and manage mouse models of obesity and diabetes. Core C is led by a group of investigators with strengths and expertise in all of the essential core activities. In order to standardize populations of megakaryocytes and platelets among studies, Core C will isolate and culture megakaryocytes and platelets for all U2M2-TRCT investigators. In this regard. Core C will communicate directly with Core B to ensure seamless isolation of platelets from patients with obesity and diabetes. The Core will also directly communicate with projects 1 and 2 for cell isolation, metabolic phenotyping, and related functional analyses. The Core is equipped with a cell sorter and sterile incubators for isolation and culture of purified human and mouse megakaryocytes and platelets. In addition, the core has equipment for in vitro and in vivo analyses of platelet function and metabolic phenotyping of mice that will be used in projects 1 and 2. Core investigators will also work together to generate, breed, and maintain colonies of mice that are proposed in projects 1 and 2. The U2M2-TRCT program will use the expertise and resources provided by Core C to guide and direct studies proposed in projects 1-4.
Patients with type 2 diabetes, obesity, or the metabolic syndrome are at increased risk for blood clots (thrombosis) caused by cells called platelets. Our studies will determine how metabolic factors in the blood and tissues (the metabolic milieu), such as high glucose and lipids, make platelets more prone to induce thrombosis, providing new insights into the treatment and management of diabetes and obesity.
|Rondina, Matthew T; Tatsumi, Kohei; Bastarache, Julie A et al. (2016) Microvesicle Tissue Factor Activity and Interleukin-8 Levels are Associated with Mortality in Patients with Influenza A/H1N1 Infection. Crit Care Med 44:e574-8|
|Freedman, Jane E; Gerstein, Mark; Mick, Eric et al. (2016) Diverse human extracellular RNAs are widely detected in human plasma. Nat Commun 7:11106|
|Yoo, Jae Hyuk; Shi, Dallas S; Grossmann, Allie H et al. (2016) ARF6 Is an Actionable Node that Orchestrates Oncogenic GNAQ Signaling in Uveal Melanoma. Cancer Cell 29:889-904|
|Yost, Christian C; Schwertz, HansjÃ¶rg; Cody, Mark J et al. (2016) Neonatal NET-inhibitory factor and related peptides inhibit neutrophil extracellular trap formation. J Clin Invest 126:3783-3798|
|Koliopoulou, Antigone; McKellar, Stephen H; Rondina, Matthew et al. (2016) Bleeding and thrombosis in chronic ventricular assist device therapy: focus on platelets. Curr Opin Cardiol 31:299-307|
|Stubblefield, William B; Alves, Nathan J; Rondina, Matthew T et al. (2016) Variable Resistance to Plasminogen Activator Initiated Fibrinolysis for Intermediate-Risk Pulmonary Embolism. PLoS One 11:e0148747|
|Shih, Lauren; Kaplan, David; Kraiss, Larry W et al. (2016) Platelet-Monocyte Aggregates and C-Reactive Protein are Associated with VTE in Older Surgical Patients. Sci Rep 6:27478|
|AraÃºjo, ClÃ¡udia V; Campbell, Clarissa; GonÃ§alves-de-Albuquerque, Cassiano F et al. (2016) A PPARÎ³ AGONIST ENHANCES BACTERIAL CLEARANCE THROUGH NEUTROPHIL EXTRACELLULAR TRAP FORMATION AND IMPROVES SURVIVAL IN SEPSIS. Shock 45:393-403|
|Rowley, Jesse W; Chappaz, StÃ©phane; Corduan, AurÃ©lie et al. (2016) Dicer1-mediated miRNA processing shapes the mRNA profile and function of murine platelets. Blood 127:1743-51|
|Beaulieu, Lea M; Vitseva, Olga; Tanriverdi, Kahraman et al. (2016) Platelet functional and transcriptional changes induced by intralipid infusion. Thromb Haemost 115:1147-56|
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