The Glycobiology Core will provide a resource for all Center members providing expertise and facilities in glycan/glycoprotein engineering and structural analysis of glycans. Approximately half of the molecular mass of HIV gp120 is comprised of N-linked glycans that shield the protein backbone. These glycans impact on vaccine design in three distinct ways. Firstly, the carbohydrates themselves are distinct from typical human glycosylation and can serve as a target for broadly neutralizing antibodies. Secondly, the glycans of gp120 limit or modulate antibody recognition of underlying protein epitopes. Finally, both viral (and immunogen) glycans can interact with host cell lectins and trigger major immunomodulatory signaling pathways. The Glycobiology Core is devoted to supporting Center members in the optimization of these critical parameters for vaccine design.
The Glycobiology Core will contribute to the development of immunogens by the provision of a range of analytical tools (NP-HPLC, MALDI-MS, ESI-MS/MS and Ion Mobility MS) and provide full compositional and linkage information on viral and immunogen glycans.
|Pauthner, Matthias; Havenar-Daughton, Colin; Sok, Devin et al. (2017) Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches. Immunity 46:1073-1088.e6|
|Mayer, Christian T; Gazumyan, Anna; Kara, Ervin E et al. (2017) The microanatomic segregation of selection by apoptosis in the germinal center. Science 358:|
|Richard, Jonathan; Prévost, Jérémie; von Bredow, Benjamin et al. (2017) BST-2 Expression Modulates Small CD4-Mimetic Sensitization of HIV-1-Infected Cells to Antibody-Dependent Cellular Cytotoxicity. J Virol 91:|
|Andrabi, Raiees; Su, Ching-Yao; Liang, Chi-Hui et al. (2017) Glycans Function as Anchors for Antibodies and Help Drive HIV Broadly Neutralizing Antibody Development. Immunity 47:1004|
|Sok, Devin; Le, Khoa M; Vadnais, Melissa et al. (2017) Rapid elicitation of broadly neutralizing antibodies to HIV by immunization in cows. Nature 548:108-111|
|Polonskaya, Zinaida; Deng, Shenglou; Sarkar, Anita et al. (2017) T cells control the generation of nanomolar-affinity anti-glycan antibodies. J Clin Invest 127:1491-1504|
|Zeltina, Antra; Krumm, Stefanie A; Sahin, Mehmet et al. (2017) Convergent immunological solutions to Argentine hemorrhagic fever virus neutralization. Proc Natl Acad Sci U S A 114:7031-7036|
|Baxter, Amy E; Niessl, Julia; Fromentin, Rémi et al. (2017) Multiparametric characterization of rare HIV-infected cells using an RNA-flow FISH technique. Nat Protoc 12:2029-2049|
|Guenaga, Javier; Garces, Fernando; de Val, Natalia et al. (2017) Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein. Immunity 46:792-803.e3|
|Bale, Shridhar; Goebrecht, Geraldine; Stano, Armando et al. (2017) Covalent Linkage of HIV-1 Trimers to Synthetic Liposomes Elicits Improved B Cell and Antibody Responses. J Virol 91:|
Showing the most recent 10 out of 276 publications