During fiscal year 2010 we accomplished the following: 1) We completed in vivo B cell priming experiments in collaboration with Dr. Mark Shlomchik (Yale University). We used mIgM-Tg mice;these mice carry a VH186.2 IgH transgene that lacks secretory exons bred to JH- and JK-deficient background. mIgM-Tg mice therefore express transgenic IgH protein complexed to lambda light chain. Approximately half the B cells in this mouse (that express Vλ1) bind the hapten NP. We injected mice with NP-BSA in PBS or PBS alone. To recapitulate pulse BCR activation, we isolated splenic B cells 2h after immunization, rested them for 4h, and re-stimulated with anti-CD40. By gating on antigen binding cells during flow cytometry we found that 2h antigen exposure in vivo enhanced the responsiveness of only antigen-specific cells to CD40 stimulation. Analysis of mRNA from short-term in vivo immunized B cells showed increased expression of several pulse-specific genes identified in the in vitro experiments. Our observations lead to a new working model for the initiation of T-dependent immune responses. 2) We completed our studies of c-Rel function during two phases of BCR-induced NF-κB activation in collaboration with Dr. Steve Gerondakis (Burnet Institute, Australia). 3) We initiated analyses of time-dependent gene expression profiling in B cells with the objective of understanding the function of NF-κB during G1 progression of B cells in response to BCR signaling. We used BCAP-deficient and PKCβ-deficient B cells to interrupt NF-κB activation. Gene expression profiling revealed a 30% overlap between genes that were down-regulated in each of these genotypes. We hypothesize that NF-κB target genes are a subset of these genes. Interestingly, pathway analysis of gene expression changes in these mutant backgrounds revealed that many 'immune response"""""""" associated pathways were up-regulated. These observations suggest that NF-κB proteins may suppress immune response pathways and accentuate cell-cycle progression pathways during early phases of T-independent immune responses.
| Lee-Chang, Catalina; Bodogai, Monica; Moritoh, Kanako et al. (2016) Aging Converts Innate B1a Cells into Potent CD8+ T Cell Inducers. J Immunol 196:3385-97 |
| Kaileh, Mary; Vazquez, Estefania; MacFarlane 4th, Alexander W et al. (2016) mTOR-Dependent and Independent Survival Signaling by PI3K in B Lymphocytes. PLoS One 11:e0146955 |
| Fowler, Trent; Garruss, Alexander S; Ghosh, Amalendu et al. (2015) Divergence of transcriptional landscape occurs early in B cell activation. Epigenetics Chromatin 8:20 |
| Maul, Robert W; Cao, Zheng; Venkataraman, Lakshmi et al. (2014) Spt5 accumulation at variable genes distinguishes somatic hypermutation in germinal center B cells from ex vivo-activated cells. J Exp Med 211:2297-306 |
| Kaileh, Mary; Sen, Ranjan (2012) NF-ýýB function in B lymphocytes. Immunol Rev 246:254-71 |
| Sen, Ranjan (2011) The origins of NF-?B. Nat Immunol 12:686-8 |
| Olkhanud, Purevdorj B; Damdinsuren, Bazarragchaa; Bodogai, Monica et al. (2011) Tumor-evoked regulatory B cells promote breast cancer metastasis by converting resting CD4? T cells to T-regulatory cells. Cancer Res 71:3505-15 |
| Fowler, Trent; Sen, Ranjan; Roy, Ananda L (2011) Regulation of primary response genes. Mol Cell 44:348-60 |
| Damdinsuren, Bazarragchaa; Zhang, Yongqing; Khalil, Ashraf et al. (2010) Single round of antigen receptor signaling programs naive B cells to receive T cell help. Immunity 32:355-66 |
| Sen, Ranjan; Smale, Stephen T (2010) Selectivity of the NF-{kappa}B response. Cold Spring Harb Perspect Biol 2:a000257 |
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