Abstract

Previously, we found that chromatin remodeling was required for Th2 cytokine gene expression. We found that transcription factors recruited BRG1 to specific sites in Th2 cells. BRG1 was required before and after Th2 differentiation. Progress: We identified the BAF version of the SWI-SNF complex as a regulator of Th2 gene expression. We found BRG1 binding sites in stimulated Th2 cells were different than in resting Th2 cells or stimulated Th1 cells. We found preliminary evidence for the involvement of SWI/SNF in other Th fates. Future: Remodeling and other cytokine loci. Remodeling and other T cell fates.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAG000524-05
Application #
7963986
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
2009
Total Cost
$480,161
Indirect Cost

  Institution

Name
National Institute on Aging
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Wurster, Andrea L; Pazin, Michael J (2012) ATP-dependent chromatin remodeling in T cells. Biochem Cell Biol 90:1-13
Wurster, Andrea L; Precht, Patricia; Becker, Kevin G et al. (2012) IL-10 transcription is negatively regulated by BAF180, a component of the SWI/SNF chromatin remodeling enzyme. BMC Immunol 13:9
Wurster, Andrea L; Precht, Patricia; Pazin, Michael J (2011) NF-?B and BRG1 bind a distal regulatory element in the IL-3/GM-CSF locus. Mol Immunol 48:2178-88
De, Supriyo; Wurster, Andrea L; Precht, Patricia et al. (2011) Dynamic BRG1 recruitment during T helper differentiation and activation reveals distal regulatory elements. Mol Cell Biol 31:1512-27
Precht, Patricia; Wurster, Andrea L; Pazin, Michael J (2010) The SNF2H chromatin remodeling enzyme has opposing effects on cytokine gene expression. Mol Immunol 47:2038-46
Mandinova, Anna; Kolev, Vihren; Neel, Victor et al. (2009) A positive FGFR3/FOXN1 feedback loop underlies benign skin keratosis versus squamous cell carcinoma formation in humans. J Clin Invest 119:3127-37
Ishii, Haruhiko; Du, Hansen; Zhang, Zhaoqing et al. (2009) Mi2beta shows chromatin enzyme specificity by erasing a DNase I-hypersensitive site established by ACF. J Biol Chem 284:7533-41
Wurster, Andrea L; Pazin, Michael J (2008) BRG1-mediated chromatin remodeling regulates differentiation and gene expression of T helper cells. Mol Cell Biol 28:7274-85