Mutations in genes coding for Fanconi anemia (FA) proteins lead to FA with early mortality. We and others have found that FA proteins associate with telomeres and their loss impacts telomere length. We hypothesize that FA proteins regulate telomere length by resolving unique telomere DNA structures. To date, at least fifteen FA genes have been identified and are functionally grouped into three categories: a core complex, the ID complex, and the downstream effectors. It has been shown that a downstream effector, SLX4 interacts with the telomere repeat binding factor, TRF2 in human cells. Currently, we are in the process of investigating the role of SLX4 in telomere length regulation. My collaborator, Dr. Ming Lei at Shanghai Institutes for Biological Sciences has solved the crystal structure of SLX4-TRF2 complex, while my lab has found that SLX4 recruits structure-specific endonucleases, XPF, MUS81, and SLX1 to telomeres via its interaction with TRF2. The SLX4-nuclease-TRF2 complex leads to rapid telomere loss (or telomere trimming) in human cells. Using in vitro and in vivo approaches, my lab has identified molecular mechanisms of SLX4 in telomere trimming, in which SLX4-nuclease-TRF2 complex is involved in the nucleolytic resolution of T-loop and telomere recombination intermediates. Thus, SLX4, together with TRF2, functions as a scaffold to recruit various endonucleases to telomeres for recombination-based telomere maintenance. For the long-term goal, we plan to investigate if SLX4 and its interacting nucleases might initiate telomere loss and contributing to human disease, such as FA.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Investigator-Initiated Intramural Research Projects (ZIA)
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National Institute on Aging
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Sarkar, Jaya; Liu, Yie (2016) Fanconi anemia proteins in telomere maintenance. DNA Repair (Amst) 43:107-12
Sarkar, Jaya; Liu, Yie (2016) The origin of oxidized guanine resolves the puzzle of oxidation-induced telomere-length alterations. Nat Struct Mol Biol 23:1070-1071
Yin, Jinhu; Wan, Bingbing; Sarkar, Jaya et al. (2016) Dimerization of SLX4 contributes to functioning of the SLX4-nuclease complex. Nucleic Acids Res 44:4871-80
Sarkar, Jaya; Wan, Bingbing; Yin, Jinhu et al. (2015) SLX4 contributes to telomere preservation and regulated processing of telomeric joint molecule intermediates. Nucleic Acids Res 43:5912-23
Popuri, Venkateswarlu; Hsu, Joseph; Khadka, Prabhat et al. (2014) Human RECQL1 participates in telomere maintenance. Nucleic Acids Res 42:5671-88
Lu, Jian; Vallabhaneni, Haritha; Yin, Jinhu et al. (2013) Deletion of the major peroxiredoxin Tsa1 alters telomere length homeostasis. Aging Cell 12:635-44
Wan, Bingbing; Yin, Jinhu; Horvath, Kent et al. (2013) SLX4 assembles a telomere maintenance toolkit by bridging multiple endonucleases with telomeres. Cell Rep 4:861-9
Ghosh, Avik K; Rossi, Marie L; Singh, Dharmendra Kumar et al. (2012) RECQL4, the protein mutated in Rothmund-Thomson syndrome, functions in telomere maintenance. J Biol Chem 287:196-209