We will see if ABCs have encountered antigen and undergone somatic hypermutation and class switching. Diversity in the repertoire and the mutation frequency will be examined in old mice. This may determine if the old cells are exhausted from chronic antigen exposure. We will see if DNA repair affects the production of ABCs by using mice deficient for repair enzymes. We will also measure the frequency of chromosome translocations in these cells, to study the mechanism of how aging produces cancer.
|Russell Knode, Lisa M; Naradikian, Martin S; Myles, Arpita et al. (2017) Age-Associated B Cells Express a Diverse Repertoire of VH and V? Genes with Somatic Hypermutation. J Immunol 198:1921-1927|
|Jones, Bart G; Penkert, Rhiannon R; Xu, Beisi et al. (2016) Binding of estrogen receptors to switch sites and regulatory elements in the immunoglobulin heavy chain locus of activated B cells suggests a direct influence of estrogen on antibody expression. Mol Immunol 77:97-102|
|Hurwitz, Julia L; Penkert, Rhiannon R; Xu, Beisi et al. (2016) Hotspots for Vitamin-Steroid-Thyroid Hormone Response Elements Within Switch Regions of Immunoglobulin Heavy Chain Loci Predict a Direct Influence of Vitamins and Hormones on B Cell Class Switch Recombination. Viral Immunol 29:132-6|
|Vallabhaneni, Haritha; Zhou, Fang; Maul, Robert W et al. (2015) Defective repair of uracil causes telomere defects in mouse hematopoietic cells. J Biol Chem 290:5502-11|
|Maul, Robert W; Gearhart, Patricia J (2014) Refining the Neuberger model: Uracil processing by activated B cells. Eur J Immunol 44:1913-6|