We have completed RAGE dimerization/oligomerization studies and a manuscript has been published. We continue to collaborate with colleagues in Australia to study RAGE thiol-disulfide bond exchange on the cell surface. Proteomics data showed that RAGE is acetylated. We are working with Johns Hopkins colleagues to study how such modification impact RAGE signaling and/or biology. We have recently deciphered the AGE-induced NF-kappaB RelA barcode, or signal-specific post-translational modifications, and linked the barcode to collagen I transcriptions in various cell types. Increased collagen content is a hallmark for aging-associated structural and functional changes in the vasculature. Our work, for the first time, established a direct link between AGE-RAGE axis and vascular aging phenotype. We are also actively studying the mechanism of RAGE shedding and trafficking in the cell, as well as spatiotemporal role of RAGE in signaling.
| Peng, Yunqian; Kim, Ji-Min; Park, Hal-Sol et al. (2016) AGE-RAGE signal generates a specific NF-?B RelA ""barcode"" that directs collagen I expression. Sci Rep 6:18822 |
| Low, Daren; Subramaniam, Renuka; Lin, Li et al. (2015) Chitinase 3-like 1 induces survival and proliferation of intestinal epithelial cells during chronic inflammation and colitis-associated cancer by regulating S100A9. Oncotarget 6:36535-50 |
| Wei, Wen; Lampe, Leonie; Park, Sungha et al. (2012) Disulfide bonds within the C2 domain of RAGE play key roles in its dimerization and biogenesis. PLoS One 7:e50736 |
