In FY 2013, we continued investigation of the staphylococcal peptide cytolysins, phenol-soluble modulins (PSMs). Current efforts focus on structure-function relationship studies of PSMs, antimicrobial activities of PSMs, and the PSM exporter, with the long-term goals to produce anti-PSM therapeutics for the treatment of staphylococcal infections. We have identified the PSM exporter and are currently setting up translational follow-up projects to find drugs interfering with PSM export. We also produced monoclonal antibodies targeting the SasX protein, previously identified to promote colonization and infection of a variety of epidemic MRSA strains in China. These will be tested in the future for a potential inhibition of colonization/infection.

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Chen, Yan; Yeh, Anthony J; Cheung, Gordon Y C et al. (2015) Basis of virulence in a Panton-Valentine leukocidin-negative community-associated methicillin-resistant Staphylococcus aureus strain. J Infect Dis 211:472-80
Cheung, Gordon Y C; Villaruz, Amer E; Joo, Hwang-Soo et al. (2014) Genome-wide analysis of the regulatory function mediated by the small regulatory psm-mec RNA of methicillin-resistant Staphylococcus aureus. Int J Med Microbiol 304:637-44
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Cheung, Gordon Y C; Kretschmer, Dorothee; Duong, Anthony C et al. (2014) Production of an attenuated phenol-soluble modulin variant unique to the MRSA clonal complex 30 increases severity of bloodstream infection. PLoS Pathog 10:e1004298
Otto, Michael (2014) Staphylococcus aureus toxins. Curr Opin Microbiol 17:32-7
Cheung, Gordon Y C; Kretschmer, Dorothee; Queck, Shu Y et al. (2014) Insight into structure-function relationship in phenol-soluble modulins using an alanine screen of the phenol-soluble modulin (PSM) *3 peptide. FASEB J 28:153-61
Otto, Michael (2014) Phenol-soluble modulins. Int J Med Microbiol 304:164-9

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