In 2012, we, in collaboration with Dr. Yuan of Virginia Tech, performed a challenge study of the recombinant subunit P2-Wa VP8 candidate vaccine in gnotobiotic piglets. Five-day-old piglets were immunized intramuscularly 3 times with the P2-Wa VP8 vaccine with aluminum phosphate adjuvant. Seven days after the 3rd immunization, both vaccinated and mock-vaccinated control piglets were challenged orally with virulent human rotavirus Wa strain. The vaccine significantly delayed the onset of diarrhea and significantly reduced duration of diarrhea and cumulative diarrhea score. The amount of infectious Wa virus shed in stools of the vaccinated piglets was substantially lower (not statistically significant) compared to the controls.
|Wen, Xiaobo; Cao, Dianjun; Jones, Ronald W et al. (2012) Construction and characterization of human rotavirus recombinant VP8* subunit parenteral vaccine candidates. Vaccine 30:6121-6|
|Feng, Ningguo; Sen, Adrish; Wolf, Marie et al. (2011) Roles of VP4 and NSP1 in determining the distinctive replication capacities of simian rotavirus RRV and bovine rotavirus UK in the mouse biliary tract. J Virol 85:2686-94|