In FY 2013, we continued our efforts to understand the impact that phenol-soluble modulins have on biofilm development. We have shown the contribution of beta-type PSMs to S. epidermidis biofilm development and all PSMs to S. aureus biofilm development in recent papers in recent papers (Wang et al. J Clin Invest 2011, Periasamy et al. PNAS 2012). We are now trying to complete our set of isogenic deletion mutants in S. epidermidis psm loci to investigate the role all PSMs play in S. epidermidis biofilm development and dissemination from device-related infection in vivo.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Zip Code
Otto, Michael (2014) Staphylococcus epidermidis pathogenesis. Methods Mol Biol 1106:17-31
Otto, Michael (2014) Physical stress and bacterial colonization. FEMS Microbiol Rev 38:1250-70
Yu, Dongliang; Pi, Borui; Chen, Yan et al. (2014) Characterization of the staphylococcal cassette chromosome composite island of Staphylococcus haemolyticus SH32, a methicillin-resistant clinical isolate from China. PLoS One 9:e87346
Joo, Hwang-Soo; Otto, Michael (2014) The isolation and analysis of phenol-soluble modulins of Staphylococcus epidermidis. Methods Mol Biol 1106:93-100
Stavrakis, Alexandra I; Niska, Jared A; Shahbazian, Jonathan H et al. (2014) Combination prophylactic therapy with rifampin increases efficacy against an experimental Staphylococcus epidermidis subcutaneous implant-related infection. Antimicrob Agents Chemother 58:2377-86
Otto, Michael (2013) Staphylococcal infections: mechanisms of biofilm maturation and detachment as critical determinants of pathogenicity. Annu Rev Med 64:175-88
Cogen, Anna L; Yamasaki, Kenshi; Sanchez, Katheryn M et al. (2010) Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin. J Invest Dermatol 130:192-200
Queck, Shu Y; Khan, Burhan A; Wang, Rong et al. (2009) Mobile genetic element-encoded cytolysin connects virulence to methicillin resistance in MRSA. PLoS Pathog 5:e1000533
Otto, Michael (2009) Staphylococcus epidermidis--the 'accidental'pathogen. Nat Rev Microbiol 7:555-67
Kuehl, Richard; Al-Bataineh, Sameer; Gordon, Oliver et al. (2009) Furanone at subinhibitory concentrations enhances staphylococcal biofilm formation by luxS repression. Antimicrob Agents Chemother 53:4159-66