Abstract

The group began in April 2009 and the projects were assigned in July 2009. We are organizing the laboratory, hiring personnel and designing experiments. The equipment and reagents are being purchased and the laboratory space adjusted for housing the mass spectrometers. We have purchased the LTQ-Orbitrap and TSQ Quantum Vantage from Thermo Fisher, and the nano-LC systems. These instruments should cover the range of initially planned experiments. We also have purchased the labeling reagents (iTRAQ from Applied Biosystems) for quantification of peptides in up to 8 samples in one experiment.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI001084-01
Application #
7964729
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2009
Total Cost
$166,802
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Khan, Mohd M; Koppenol-Raab, Marijke; Kuriakose, Minna et al. (2018) Host-pathogen dynamics through targeted secretome analysis of stimulated macrophages. J Proteomics 189:34-38
Khan, Mohd M; Ernst, Orna; Sun, Jing et al. (2018) Mass Spectrometry-based Structural Analysis and Systems Immunoproteomics Strategies for Deciphering the Host Response to Endotoxin. J Mol Biol 430:2641-2660
Manes, Nathan P; Shulzhenko, Natalia; Nuccio, Arthur G et al. (2017) Multi-omics Comparative Analysis Reveals Multiple Layers of Host Signaling Pathway Regulation by the Gut Microbiota. mSystems 2:
Koppenol-Raab, Marijke; Nita-Lazar, Aleksandra (2017) A Methodology for Comprehensive Analysis of Toll-Like Receptor Signaling in Macrophages. Methods Mol Biol 1636:301-312
Koppenol-Raab, Marijke; Sjoelund, Virginie; Manes, Nathan P et al. (2017) Proteome and Secretome Analysis Reveals Differential Post-transcriptional Regulation of Toll-like Receptor Responses. Mol Cell Proteomics 16:S172-S186
Nuccio, A G; Bui, M; Dalal, Y et al. (2017) Mass Spectrometry-Based Methodology for Identification of Native Histone Variant Modifications From Mammalian Tissues and Solid Tumors. Methods Enzymol 586:275-290
Bui, Minh; Pitman, Mary; Nuccio, Arthur et al. (2017) Internal modifications in the CENP-A nucleosome modulate centromeric dynamics. Epigenetics Chromatin 10:17
Sjoelund, Virginie; Smelkinson, Margery; Nita-Lazar, Aleksandra (2014) Phosphoproteome profiling of the macrophage response to different toll-like receptor ligands identifies differences in global phosphorylation dynamics. J Proteome Res 13:5185-97
Bui, Minh; Dimitriadis, Emilios K; Hoischen, Christian et al. (2012) Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivo. Cell 150:317-26
Germain, Ronald N; Meier-Schellersheim, Martin; Nita-Lazar, Aleksandra et al. (2011) Systems biology in immunology: a computational modeling perspective. Annu Rev Immunol 29:527-85

Showing the most recent 10 out of 13 publications