The transfer of phospholipids between the ER and mitochondria is critical for mitochondrial biogenesis. Mitochondria cannot synthesize many of the lipids they require for membrane biogenesis and yet there is little or no vesicular trafficking to mitochondria. It is thought that lipids are transferred from the ER to mitochondria by a poorly understood nonvesicular mechanism. This transport has been proposed to occur at regions where the ER and mitochondria are closely apposed. In order to identify proteins required for phospholipids exchange between the ER and mitochondria we performed a genetic screen in collaboration with Dr. Chistopher Loewen at the University of British Columbia. We identified a conserved complex of ER proteins. We measured the exchange of phosphatidylserine between the ER and mitochondria in mutants missing a number of components of this complex. We found that lipid exchange slowed about 5 fold both in vivo and in vitro. This work is being prepared for submission.

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Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
2012
Total Cost
$476,393
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Choudhary, Vineet; Golden, Andy; Prinz, William A (2016) Keeping FIT, storing fat: Lipid droplet biogenesis. Worm 5:e1170276
Michaud, Morgane; Gros, Valérie; Tardif, Marianne et al. (2016) AtMic60 Is Involved in Plant Mitochondria Lipid Trafficking and Is Part of a Large Complex. Curr Biol 26:627-39
Michaud, Morgane; Prinz, William A; Jouhet, Juliette (2016) Glycerolipid synthesis and lipid trafficking in plant mitochondria. FEBS J :
Lahiri, Sujoy; Toulmay, Alexandre; Prinz, William A (2015) Membrane contact sites, gateways for lipid homeostasis. Curr Opin Cell Biol 33:82-7
Choudhary, Vineet; Ojha, Namrata; Golden, Andy et al. (2015) A conserved family of proteins facilitates nascent lipid droplet budding from the ER. J Cell Biol 211:261-71
Murley, Andrew; Sarsam, Reta D; Toulmay, Alexandre et al. (2015) Ltc1 is an ER-localized sterol transporter and a component of ER-mitochondria and ER-vacuole contacts. J Cell Biol 209:539-48
Prinz, William A (2014) The lipid trade. Nat Rev Mol Cell Biol 15:79
Banfield, David K; Prinz, Will (2014) Editorial overview: Cell organelles. Curr Opin Cell Biol 29:v-vi
Lahiri, Sujoy; Chao, Jesse T; Tavassoli, Shabnam et al. (2014) A conserved endoplasmic reticulum membrane protein complex (EMC) facilitates phospholipid transfer from the ER to mitochondria. PLoS Biol 12:e1001969
Prinz, William A (2014) Bridging the gap: membrane contact sites in signaling, metabolism, and organelle dynamics. J Cell Biol 205:759-69

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