Genetic Analyses For Epidemiologic Studies Of Respiratory Disease

London, Stephanie

Abstract

This project describes the role of the lab of Stephanie London in the Laboratory of Respiratory Biology in support of her epidemiologic studies. The laboratory is engaged in genetic and epigenetic analyses to support Dr. Londons epidemiologic projects. In the past year, we have focused on genome wide approaches. The lab supports follow-up of findings from our genome wide association studies of childhood asthma as well as adult pulmonary function. It also supports our project to analyze genome-wide methylation platform (Illumina 450K) using cord blood samples from the Norwegian Mother and Child Study. We are examining associations with maternal exposures and childhood asthma. The lab has been doing genotyping of polymorphisms that may influence associations between exposures and epigenetic changes. We have also evaluate new commercial platforms for assessing global methylation. The lab has prepared samples for high throughput genetic and epigenetic platforms.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Environmental Health Sciences (NIEHS)
• Type: Investigator-Initiated Intramural Research Projects (ZIA)
• Project #: 1ZIAES025045-12
• Application #: 8336533
• Support Year: 12
• Fiscal Year: 2011
• Total Cost: $289,492

Institution

• Name: National Institute of Environmental Health Sciences

Publications

Nichols, Cody E; Shepherd, Danielle L; Hathaway, Quincy A et al. (2018) Reactive oxygen species damage drives cardiac and mitochondrial dysfunction following acute nano-titanium dioxide inhalation exposure. Nanotoxicology 12:32-48

House, John S; Nichols, Cody E; Li, Huiling et al. (2017) Vagal innervation is required for pulmonary function phenotype in Htr4(-/-) mice. Am J Physiol Lung Cell Mol Physiol 312:L520-L530

Obeidat, Ma'en; Hao, Ke; Bossé, Yohan et al. (2015) Molecular mechanisms underlying variations in lung function: a systems genetics analysis. Lancet Respir Med 3:782-95

Yan, Xiting; Chu, Jen-Hwa; Gomez, Jose et al. (2015) Noninvasive analysis of the sputum transcriptome discriminates clinical phenotypes of asthma. Am J Respir Crit Care Med 191:1116-25

Magnus, Maria C; Håberg, Siri E; Karlstad, Øystein et al. (2015) Grandmother's smoking when pregnant with the mother and asthma in the grandchild: the Norwegian Mother and Child Cohort Study. Thorax 70:237-43

Gharib, Sina A; Loth, Daan W; Soler Artigas, María et al. (2015) Integrative pathway genomics of lung function and airflow obstruction. Hum Mol Genet 24:6836-48

House, John S; Li, Huiling; DeGraff, Laura M et al. (2015) Genetic variation in HTR4 and lung function: GWAS follow-up in mouse. FASEB J 29:323-35

Sharma, Sunita; Kho, Alvin T; Chhabra, Divya et al. (2015) Glucocorticoid genes and the developmental origins of asthma susceptibility and treatment response. Am J Respir Cell Mol Biol 52:543-53

Reddy, Poovendhree; Naidoo, Rajen N; Robins, Thomas G et al. (2012) GSTM1 and GSTP1 gene variants and the effect of air pollutants on lung function measures in South African children. Am J Ind Med 55:1078-86

Wilk, Jemma B; Shrine, Nick R G; Loehr, Laura R et al. (2012) Genome-wide association studies identify CHRNA5/3 and HTR4 in the development of airflow obstruction. Am J Respir Crit Care Med 186:622-32