We previously showed that the 5A peptide can promote cholesterol efflux by the ABCA1 transporter and reduce atherosclerosis in animal models. The peptide was licensed to KineMed Inc, who received a RAID grant for performing preclinical toxicology studies, which have commenced. We have done additional structure function studies on the 5A peptide and identified 2 key hydrophobic residues in the hinge region of this bi-helical peptide for it to be able to promote cholesterol efflux. Based on this finding, we developed a new membrane solubilization model for how apolipoprotein mimetic peptides interact with membranes and promote cholesterol efflux.
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|Dai, Cuilian; Yao, Xianglan; Vaisman, Boris et al. (2014) The ABCA1 Transporter Attenuates Ovalbumin-induced Neutrophilic Airway Inflammation. Am J Respir Cell Mol Biol :|
|Vickers, Kasey C; Remaley, Alan T (2014) HDL and cholesterol: life after the divorce? J Lipid Res 55:4-12|
|Remaley, Alan T (2013) Apolipoprotein A-II: still second fiddle in high-density lipoprotein metabolism? Arterioscler Thromb Vasc Biol 33:166-7|
|Sviridov, Denis O; Andrianov, Alexander M; Anishchenko, Ivan V et al. (2013) Hydrophobic amino acids in the hinge region of the 5A apolipoprotein mimetic peptide are essential for promoting cholesterol efflux by the ABCA1 transporter. J Pharmacol Exp Ther 344:50-8|
|Ditiatkovski, Michael; D'Souza, Wilissa; Kesani, Rajitha et al. (2013) An apolipoprotein A-I mimetic peptide designed with a reductionist approach stimulates reverse cholesterol transport and reduces atherosclerosis in mice. PLoS One 8:e68802|