The Vaccine Research Center Nonhuman primate Immunogenicity Core supports the basic research laboratories of the VRC that are performing vaccination experiments with nonhuman primates. The Core has developed rigorous protocols for the isolation of cells, cryopreservation of cells, isolation of mucosal specimens, assays to detect immune function, and assays to determine lymphocyte representation in tissues. The NIC will qualify and validate these assays in order to provide the most rigorous support for basic research laboratories by ensuring consistency, sensitivity, and reproducibility. The Core has expertise in all aspects of NHP experiments, and provides support to investigators at multiple levels: design and implementation of experiments, writing and presenting the animal protocols to the Institutional Animal Care and Use Committee, scheduling of experiments, interfacing with the Laboratory of Animal Medicine and animal contract facilities, processing of specimens, data analysis. The Core also maintains a cryorepository of specimens, and participates in international training and collaborations to ensure translation of Core assays to other sites.

Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2011
Total Cost
$865,364
Indirect Cost
City
State
Country
Zip Code
Zurawski, Gerard; Zurawski, Sandra; Flamar, Anne-Laure et al. (2016) Targeting HIV-1 Env gp140 to LOX-1 Elicits Immune Responses in Rhesus Macaques. PLoS One 11:e0153484
Ishizuka, Andrew S; Lyke, Kirsten E; DeZure, Adam et al. (2016) Corrigendum: Protection against malaria at 1 year and immune correlates following PfSPZ vaccination. Nat Med 22:692
Ishizuka, Andrew S; Lyke, Kirsten E; DeZure, Adam et al. (2016) Protection against malaria at 1 year and immune correlates following PfSPZ vaccination. Nat Med 22:614-23
Szomolay, Barbara; Liu, Jie; Brown, Paul E et al. (2016) Identification of human viral protein-derived ligands recognized by individual MHCI-restricted T-cell receptors. Immunol Cell Biol 94:573-82
Vaccari, Monica; Gordon, Shari N; Fourati, Slim et al. (2016) Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition. Nat Med 22:762-70
Roederer, Mario (2015) Parsimonious Determination of the Optimal Infectious Dose of a Pathogen for Nonhuman Primate Models. PLoS Pathog 11:e1005100
Stanley, Daphne A; Honko, Anna N; Asiedu, Clement et al. (2014) Chimpanzee adenovirus vaccine generates acute and durable protective immunity against ebolavirus challenge. Nat Med 20:1126-9
Sambor, Anna; Garcia, Ambrosia; Berrong, Mark et al. (2014) Establishment and maintenance of a PBMC repository for functional cellular studies in support of clinical vaccine trials. J Immunol Methods 409:107-16
Donaldson, Mitzi M; Kao, Shing-Fen; Eslamizar, Leila et al. (2012) Optimization and qualification of an 8-color intracellular cytokine staining assay for quantifying T cell responses in rhesus macaques for pre-clinical vaccine studies. J Immunol Methods 386:10-21
Foulds, Kathryn E; Donaldson, Mitzi; Roederer, Mario (2012) OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells. Cytometry A 81:360-1

Showing the most recent 10 out of 13 publications