The Facility operating expenses primarily include the cost of annual instrument specific service contracts, covering two laser scanning confocal microscopes and laser microdissection microscope, and purchase of necessary equipment and software upgrades. For example, fluorophore specific fluorescence filter cubes were purchased to optimize imaging of the newly emerging genetically encoded fluorescent proteins. As new technology becomes available, novel imaging procedures are developed and continually refined to take advantage of the full capabilities of the instruments. Image processing and analysis are developed to quantify and standardize observations made using the imaging based assays. Purchasing of reagents, culture dishes, etc. are generally the responsibility of the specific user, but are borne by the facility when directly related to Dr. Kruhlak's work to develop microscope based assays. The Facility provides start up consumable reagents that are replenished by the user.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC010915-06
Application #
8763730
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2013
Total Cost
$312,071
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Polato, Federica; Callen, Elsa; Wong, Nancy et al. (2014) CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J Exp Med 211:1027-36
Wang, Pingping; Zhou, Zhihong; Hu, Anchang et al. (2014) Both decreased and increased SRPK1 levels promote cancer by interfering with PHLPP-mediated dephosphorylation of Akt. Mol Cell 54:378-91
Nguyen, Vinh-Phuc; Chen, Jing; Petrus, Michael N et al. (2014) A new domain in the Toll/IL-1R domain-containing adaptor inducing interferon-? factor protein amino terminus is important for tumor necrosis factor-? receptor-associated factor 3 association, protein stabilization and interferon signaling. J Innate Immun 6:377-93
Sousa, Alioscka A; Kruhlak, Michael J (2013) Introduction: nanoimaging techniques in biology. Methods Mol Biol 950:1-10
Massimelli, Maria J; Majerciak, Vladimir; Kruhlak, Michael et al. (2013) Interplay between polyadenylate-binding protein 1 and Kaposi's sarcoma-associated herpesvirus ORF57 in accumulation of polyadenylated nuclear RNA, a viral long noncoding RNA. J Virol 87:243-56
Kruhlak, Michael J (2013) Correlative fluorescence and EFTEM imaging of the organized components of the mammalian nucleus. Methods Mol Biol 950:397-416
Callen, Elsa; Bunting, Sam; Huang, Ching-Yu et al. (2009) Chimeric IgH-TCRalpha/delta translocations in T lymphocytes mediated by RAG. Cell Cycle 8:2408-12
Kruhlak, Michael J; Celeste, Arkady; Nussenzweig, Andre (2009) Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy. Methods Mol Biol 523:125-40
Hao, Jian-Jiang; Liu, Yin; Kruhlak, Michael et al. (2009) Phospholipase C-mediated hydrolysis of PIP2 releases ERM proteins from lymphocyte membrane. J Cell Biol 184:451-62
Jia, Rong; Liu, Xuefeng; Tao, Mingfang et al. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol 83:167-80

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