The Facility operating expenses primarily include the cost of annual instrument specific service contracts, covering two laser scanning confocal microscopes and laser microdissection microscope, and purchase of necessary equipment and software upgrades. For example, fluorophore specific fluorescence filter cubes were purchased to optimize imaging of the newly emerging genetically encoded fluorescent proteins. As new technology becomes available, novel imaging procedures are developed and continually refined to take advantage of the full capabilities of the instruments. Image processing and analysis are developed to quantify and standardize observations made using the imaging based assays. Purchasing of reagents, culture dishes, etc. are generally the responsibility of the specific user, but are borne by the facility when directly related to Dr. Kruhlak's work to develop microscope based assays. The Facility provides start up consumable reagents that are replenished by the user.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC010915-06
Application #
8763730
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2013
Total Cost
$312,071
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Sharma, Nishi R; Wang, Xiaohong; Majerciak, Vladimir et al. (2016) Cell Type- and Tissue Context-dependent Nuclear Distribution of Human Ago2. J Biol Chem 291:2302-9
Strickfaden, Hilmar; McDonald, Darin; Kruhlak, Michael J et al. (2016) Poly(ADP-ribosyl)ation-dependent Transient Chromatin Decondensation and Histone Displacement following Laser Microirradiation. J Biol Chem 291:1789-802
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Polato, Federica; Callen, Elsa; Wong, Nancy et al. (2014) CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J Exp Med 211:1027-36
Wang, Pingping; Zhou, Zhihong; Hu, Anchang et al. (2014) Both decreased and increased SRPK1 levels promote cancer by interfering with PHLPP-mediated dephosphorylation of Akt. Mol Cell 54:378-91
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Callen, Elsa; Di Virgilio, Michela; Kruhlak, Michael J et al. (2013) 53BP1 mediates productive and mutagenic DNA repair through distinct phosphoprotein interactions. Cell 153:1266-80

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