The Facility operating expenses primarily include the cost of annual instrument specific service contracts, covering two laser scanning confocal microscopes and laser microdissection microscope, and purchase of necessary equipment and software upgrades. For example, fluorophore specific fluorescence filter cubes were purchased to optimize imaging of the newly emerging genetically encoded fluorescent proteins. As new technology becomes available, novel imaging procedures are developed and continually refined to take advantage of the full capabilities of the instruments. Image processing and analysis are developed to quantify and standardize observations made using the imaging based assays. Purchasing of reagents, culture dishes, etc. are generally the responsibility of the specific user, but are borne by the facility when directly related to Dr. Kruhlak's work to develop microscope based assays. The Facility provides start up consumable reagents that are replenished by the user.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Scientific Cores Intramural Research (ZIC)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
National Cancer Institute Division of Basic Sciences
Zip Code
Sharma, Nishi R; Wang, Xiaohong; Majerciak, Vladimir et al. (2016) Cell Type- and Tissue Context-dependent Nuclear Distribution of Human Ago2. J Biol Chem 291:2302-9
Strickfaden, Hilmar; McDonald, Darin; Kruhlak, Michael J et al. (2016) Poly(ADP-ribosyl)ation-dependent Transient Chromatin Decondensation and Histone Displacement following Laser Microirradiation. J Biol Chem 291:1789-802
Bhattacharya, Parna; Dey, Ranadhir; Dagur, Pradeep K et al. (2015) Genetically Modified Live Attenuated Leishmania donovani Parasites Induce Innate Immunity through Classical Activation of Macrophages That Direct the Th1 Response in Mice. Infect Immun 83:3800-15
Kaul, Sunil; Mittal, Sharad K; Feigenbaum, Lionel et al. (2015) Expression of the SNARE protein SNAP-23 is essential for cell survival. PLoS One 10:e0118311
Williams, Joy A; Zhang, Jingjing; Jeon, Hyein et al. (2014) Thymic medullary epithelium and thymocyte self-tolerance require cooperation between CD28-CD80/86 and CD40-CD40L costimulatory pathways. J Immunol 192:630-40
Nguyen, Vinh-Phuc; Chen, Jing; Petrus, Michael N et al. (2014) A new domain in the Toll/IL-1R domain-containing adaptor inducing interferon-β factor protein amino terminus is important for tumor necrosis factor-α receptor-associated factor 3 association, protein stabilization and interferon signaling. J Innate Immun 6:377-93
Polato, Federica; Callen, Elsa; Wong, Nancy et al. (2014) CtIP-mediated resection is essential for viability and can operate independently of BRCA1. J Exp Med 211:1027-36
Wang, Pingping; Zhou, Zhihong; Hu, Anchang et al. (2014) Both decreased and increased SRPK1 levels promote cancer by interfering with PHLPP-mediated dephosphorylation of Akt. Mol Cell 54:378-91
Lamy, Laurence; Ngo, Vu N; Emre, N C Tolga et al. (2013) Control of autophagic cell death by caspase-10 in multiple myeloma. Cancer Cell 23:435-49
Callen, Elsa; Di Virgilio, Michela; Kruhlak, Michael J et al. (2013) 53BP1 mediates productive and mutagenic DNA repair through distinct phosphoprotein interactions. Cell 153:1266-80

Showing the most recent 10 out of 35 publications