The Surgery Branch Vector Production Facility (SBVPF) was established to provide clinical-grade retroviral and lentiviral vectors to support of our gene therapy clinical trials with the goal of providing GMP quality products while reducing production time and cost. These products, both retroviral and lentiviral vectors will be used to introduce novel T cell receptors (TCR) or chimeric antigen receptors (CAR) to genetically modify naive T cells to make them specifically recognize and kill tumor. This lab provides all the clinical reagents for our clinical gene therapy program. For this report, SBVPF currently has manufactured a murine NY-ESO-1 TCR, a DP4-retricted anti-MAGE-A3 TCR, as well as, a second production of our CD19 CAR. There are several products in production including a CSPG4 CAR, a HPV TCR, a CD27 receptor as well as an iCaspase9 suicide vector in production. Our laboratory has also provided cloning services to our group. We have constructed 12 lentiviral vectors using gateway technology for cell reprogramming. We have also developed new clinical retroviral and lentiviral vector backbones to facilitate our clinical reagent program. We are also generating 13 adenoviral vector constructs. In addition to our reagent development and production efforts, my laboratory is also involved in basic research to identify novel targets for immunotherapy. We are exploring two additional targets which involves expression screening, TCR cloning and functional analyses to assess whether these new targets are viable options for adoptive cell transfer studies.
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|Abate-Daga, Daniel; Lagisetty, Kiran H; Tran, Eric et al. (2014) A Novel Chimeric Antigen Receptor Against Prostate Stem Cell Antigen Mediates Tumor Destruction in a Humanized Mouse Model of Pancreatic Cancer. Hum Gene Ther :|
|Casati, Anna; Varghaei-Nahvi, Azam; Feldman, Steven Alexander et al. (2013) Clinical-scale selection and viral transduction of human naive and central memory CD8(+) T cells for adoptive cell therapy of cancer patients. Cancer Immunol Immunother 62:1563-73|
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|Kochenderfer, James N; Feldman, Steven A; Zhao, Yangbing et al. (2009) Construction and preclinical evaluation of an anti-CD19 chimeric antigen receptor. J Immunother 32:689-702|
|Robbins, Paul F; Li, Yong F; El-Gamil, Mona et al. (2008) Single and dual amino acid substitutions in TCR CDRs can enhance antigen-specific T cell functions. J Immunol 180:6116-31|