Abstract

The Biotechnology Lab finished its collaboration work with the NICHD on the development of conjugated vaccines against Shigella flexneri, Shigella Sonnie, Bortedella pertussis Haemophilus influenzae type A, Bortedella pertussis, and protein vaccines for Bacillus anthracis and Malaria transmission blocking vaccine. Working together with scientists from the NIAID the laboratory is evaluating the potential production of rotavirus vaccine form suspension cells. The current vaccine production procedure is based on cells growing on solid surface which is cumbersome and work intensive. Production the virus from cells growing in suspension is simpler but involves cell and virus adaptation which where we currently investigating. Concerning the recombinant vaccines initiative the lab is collaborating with the FDA on production of polysaccharides from E. coli.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICDK070011-08
Application #
8940146
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst Diabetes/Digst/Kidney
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Robbins, John B; Schneerson, Rachel; Kubler-Kielb, Joanna et al. (2014) Toward a new vaccine for pertussis. Proc Natl Acad Sci U S A 111:3213-6
Battistel, Marcos D; Shangold, Michael; Trinh, Loc et al. (2012) Evidence for helical structure in a tetramer of ?2-8 sialic acid: unveiling a structural antigen. J Am Chem Soc 134:10717-20
Bellanti, Joseph A; Lin, Feng-Ying C; Chu, Chiayung et al. (2012) Phase 1 study of a recombinant mutant protective antigen of Bacillus anthracis. Clin Vaccine Immunol 19:140-5
Cui, Xizhong; Su, Junwu; Li, Yan et al. (2010) Bacillus anthracis cell wall produces injurious inflammation but paradoxically decreases the lethality of anthrax lethal toxin in a rat model. Intensive Care Med 36:148-56
Chu, Chia; Lugovtsev, Vladimir; Lewis, Andrew et al. (2010) Production and antigenic properties of influenza virus from suspension MDCK-siat7e cells in a bench-scale bioreactor. Vaccine :
Passwell, Justen H; Ashkenzi, Shai; Banet-Levi, Yonit et al. (2010) Age-related efficacy of Shigella O-specific polysaccharide conjugates in 1-4-year-old Israeli children. Vaccine 28:2231-5
Robbins, John B; Kubler-Kielb, Joanna; Vinogradov, Evguenii et al. (2009) Synthesis, characterization, and immunogenicity in mice of Shigella sonnei O-specific oligosaccharide-core-protein conjugates. Proc Natl Acad Sci U S A 106:7974-8
Biesova, Zuzana; Miller, Mark A; Schneerson, Rachel et al. (2009) Preparation, characterization, and immunogenicity in mice of a recombinant influenza H5 hemagglutinin vaccine against the avian H5N1 A/Vietnam/1203/2004 influenza virus. Vaccine 27:6234-8