Dissecting complex disease has become more feasible due to the availability of large-scale DNA resources and advances in high-throughput genomic technology. While these tools help scientists identify potential susceptibility loci, subjects with very specific relevant genotypes are needed for clinical phenotyping and toxicity studies. The Molecular Genetics Core Facility (MGCF) infrastructure was created to perform nucleic acid sample isolation, sample management and genetics studies to support genetics efforts at NIEHS. We continue to support automated gene resequencing infrastructure, which provides support from primer design through data analysis, while dramatically reducing cost per reaction. We also continue to utilize an automated gel-free mouse tail genotyping method to reduce labor and expense of this time consuming and costly aspect of maintaining mouse colonies. Both the methods automation and methods development aspects of our work group offer opportunities for accelerated development of new therapies for prevention of environmentally induced disease.

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Lai, Lihua; Azzam, Kathleen M; Lin, Wan-Chi et al. (2016) MicroRNA-33 Regulates the Innate Immune Response via ATP Binding Cassette Transporter-mediated Remodeling of Membrane Microdomains. J Biol Chem 291:19651-60
Cheng, Jennifer; Dackor, Ryan T; Bradbury, J Alyce et al. (2016) Contribution of alveolar type II cell-derived cyclooxygenase-2 to basal airway function, lung inflammation, and lung fibrosis. FASEB J 30:160-73
Davis, Felicity M; Goulding, Eugenia H; D'Agostin, Diane M et al. (2016) Male infertility in mice lacking the store-operated Ca(2+) channel Orai1. Cell Calcium 59:189-97
House, John S; Li, Huiling; DeGraff, Laura M et al. (2015) Genetic variation in HTR4 and lung function: GWAS follow-up in mouse. FASEB J 29:323-35
Clement, Tracy M; Inselman, Amy L; Goulding, Eugenia H et al. (2015) Disrupting Cyclin Dependent Kinase 1 in Spermatocytes Causes Late Meiotic Arrest and Infertility in Mice. Biol Reprod 93:137
Gowdy, K M; Madenspacher, J H; Azzam, K M et al. (2015) Key role for scavenger receptor B-I in the integrative physiology of host defense during bacterial pneumonia. Mucosal Immunol 8:559-71
McAlees, J W; Whitehead, G S; Harley, I T W et al. (2015) Distinct Tlr4-expressing cell compartments control neutrophilic and eosinophilic airway inflammation. Mucosal Immunol 8:863-73
Van, Christopher; Williams, Jessica S; Kunkel, Thomas A et al. (2015) Deposition of histone H2A.Z by the SWR-C remodeling enzyme prevents genome instability. DNA Repair (Amst) 25:9-14
Hsia, B J; Whitehead, G S; Thomas, S Y et al. (2015) Trif-dependent induction of Th17 immunity by lung dendritic cells. Mucosal Immunol 8:186-97
Romero, Freddy; Shah, Dilip; Duong, Michelle et al. (2015) A pneumocyte-macrophage paracrine lipid axis drives the lung toward fibrosis. Am J Respir Cell Mol Biol 53:74-86

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