This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Proteomics is an emerging area of research that has been applied successfully to identify cellular biomarkers of disease progression. Ciphergen's ProteinChip? technology is an important component of the proteomics platform for the rapid discovery of multiple biomarkers when used with HPLC, FPLC and LC/MS/MS for identification of proteins. Statistics/Bioinformatics is also necessary to complement the proteomics platform that has been established at University of Puerto Rico Medical Sciences Campus (UPR MSC) in collaboration with experts at University of Nebraska Medical Center (UNMC). The long term goal of this Core Facility is to enhance our institutional biomedical research capability by establishing the necessary platform and expertise in proteomics to basic and clinical projects related to biomarker discovery. The following specific aims are proposed: 1. To provide the infrastructure necessary to characterize, and isolate differentially expressed proteins in populations with health disparities. 2. To increase the capabilities of performing research projects in proteomics among faculty and graduate students at the UPR-MSC. 3.To maintain a state of the art proteomic Core laboratory available for faculty and students and provide advice, service and training of proteomics analyses using diverse approaches in accordance to each biological system. 4.To prepare the institution for protein identification and analysis by fostering collaboration of the Biostatisticians in the mainland. This research infrastructure will be used by the UPR-MSC investigators who have started using the SELDI-TOF in pilot projects that affect our population in the areas of HIV Disease, Cancer, Diabetes, Cardiovascular diseases, Genetics and Infectious diseases. Since Protein Discovery requires sophisticated equipments, we propose to develop a Proteomics Core Facility with the minimum necessary instruments and technical expertise to provide access to investigators at UPR-MSC and increase their productivity. The relevance of this Activity is the reduction in Health Disparities by enhancing our biomedical research capability in the diseases that affect minority populations including women.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Research Centers in Minority Institutions Award (G12)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-RI-B (02))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Puerto Rico Med Sciences
Schools of Medicine
San Juan
United States
Zip Code
Rullán-Lind, Carlos; Pietri, Ruth B; Vázquez-Cintrón, Melvin et al. (2018) Fused dimerization increases expression, solubility, and activity of bacterial dehydratase enzymes. Protein Sci 27:969-975
Martínez-Rivera, Freddyson J; Barreto-Estrada, Jennifer L (2018) Reply to: Does High-Frequency Deep Brain Stimulation in Dorsal Regions of the Ventral Striatum Impair Extinction of Morphine-Induced Place Preference? Biol Psychiatry 83:e21
Ebersole, J L; Dawson 3rd, D A; Emecen Huja, P et al. (2018) Age and Periodontal Health - Immunological View. Curr Oral Health Rep 5:229-241
Ebersole, Jeffrey L; Orraca, Luis; Kensler, Terry B et al. (2018) Periodontal disease susceptible matrilines in the Cayo Santiago Macaca mulatta macaques. J Periodontal Res :
Ramírez, Maite; Santos, Saritza; Martínez, Osmarie et al. (2018) Characterization of the immune response elicited by the vaccinia virus L3 protein delivered as naked DNA. Vaccine 36:2049-2055
Vaquer-Alicea, Ana Del C; Vázquez-Torres, Rafael; Devarie-Hornedo, Marcos et al. (2018) aPKC-Mediated Persistent Increase in AMPA/NMDA Ratio in the VTA Participates in the Neuroadaptive Signal Necessary to Induce NAc Synaptic Plasticity After Cocaine Administration. Neuroscience 392:129-140
Roche-Lima, Abiel; Carrasquillo-Carrión, Kelvin; Gómez-Moreno, Ramón et al. (2018) The Presence of Genotoxic and/or Pro-inflammatory Bacterial Genes in Gut Metagenomic Databases and Their Possible Link With Inflammatory Bowel Diseases. Front Genet 9:116
Colón-Cruz, Luis; Kristofco, Lauren; Crooke-Rosado, Jonathan et al. (2018) Alterations of larval photo-dependent swimming responses (PDR): New endpoints for rapid and diagnostic screening of aquatic contamination. Ecotoxicol Environ Saf 147:670-680
Anderson, Julia; Seol, Haeri; Gordish-Dressman, Heather et al. (2017) Interleukin 1 Receptor-Like 1 Protein (ST2) is a Potential Biomarker for Cardiomyopathy in Duchenne Muscular Dystrophy. Pediatr Cardiol 38:1606-1612
Martínez, Osmarie; Bravo Cruz, Ariana; Santos, Saritza et al. (2017) Vaccination with a codon-optimized A27L-containing plasmid decreases virus replication and dissemination after vaccinia virus challenge. Vaccine 35:6007-6014

Showing the most recent 10 out of 414 publications