This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Alu elements are primate-specific short interspersed elements (SINEs). Each Alu is approximately 300 bp in length and derives its name from a single recognition site for the restriction enzyme AluI. Comprising roughly 11% of the human genome, Alu elements have amplified to more than one million copies in primate genomes over the last 65 million years, and a series of Alu subfamilies of different ages has been generated. Although Alu elements have no known biological function, the propagation of Alus has contributed a great deal to the evolution, structure, and dynamics of the human genome, and a significant proportion of human genetic disease has been ascribed to the disruptive Alu insertions and mutations. The goal of the proposed research is to develop the first customized data mining framework that can computationally characterize preferences of Alu insertion site on a broader range of base pairs, and intellectually assist hunting down disease-causing Alu insertion mutations. In particular, our specific aims are:
Aim 1. To develop an effective, scalable and unbiased discriminative data mining framework for Alu insertion site prediction. Three inevitable and distinct components that will be investigated are defined as follows: i. Specialized feature generation methods for Alu sequence data; ii. A divide-and-conquer based feature selection and refinement mechanism which is driven by frequent-itemset mining;and iii. A scalable and unbiased discriminative model augmented by probabilistic tree ensembles.
Aim 2. To design a biological testing framework with increased focus on thoroughly validating proposed Alu insertion site prediction model through phylogenetic footprinting. The proposed study will assist not only in understanding Alu elements themselves and their effects in human genetic diseases, but also in integrating and developing a series of advanced data mining techniques for biological sequence analysis. Significant progress towards this goal will contribute to the overall recognition of Alu biology and genetic basis of human diseases.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Research Centers in Minority Institutions Award (G12)
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Xavier University of Louisiana
Other Domestic Higher Education
New Orleans
United States
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Graves, Richard A; Ledet, Grace; Nation, Cedric A et al. (2015) An ultra-high performance chromatographic method for the determination of artemisinin. Drug Dev Ind Pharm 41:819-24
Bratton, Melyssa R; Martin, Elizabeth C; Elliott, Steven et al. (2015) Glyceollin, a novel regulator of mTOR/p70S6 in estrogen receptor positive breast cancer. J Steroid Biochem Mol Biol 150:17-23
Dougherty, Casey A; Furgal, Joseph C; van Dongen, Mallory A et al. (2014) Isolation and characterization of precise dye/dendrimer ratios. Chemistry 20:4638-45
Nilov, Denis; Kucheryavy, Pavel; Walker, Verina et al. (2014) Synthesis of 5-Substituted Derivatives of Isophthalic Acid as Non-Polymeric Amphiphilic Coating for Metal Oxide Nanoparticles. Tetrahedron Lett 55:5078-5081
Strong, Amy L; Ohlstein, Jason F; Jiang, Quan et al. (2014) Novel daidzein analogs enhance osteogenic activity of bone marrow-derived mesenchymal stem cells and adipose-derived stromal/stem cells through estrogen receptor dependent and independent mechanisms. Stem Cell Res Ther 5:105
Ponnapakam, Adharsh P; Liu, Jiawang; Bhinge, Kaustubh N et al. (2014) 3-Ketone-4,6-diene ceramide analogs exclusively induce apoptosis in chemo-resistant cancer cells. Bioorg Med Chem 22:1412-20
McFerrin, Harris E; Olson, Scott D; Gutschow, Miriam V et al. (2014) Rapidly self-renewing human multipotent marrow stromal cells (hMSC) express sialyl Lewis X and actively adhere to arterial endothelium in a chick embryo model system. PLoS One 9:e105411
Ledet, Grace; Pamujula, Sarala; Walker, Valencia et al. (2014) Development and in vitro evaluation of a nanoemulsion for transcutaneous delivery. Drug Dev Ind Pharm 40:370-9
Abboud, Elizabeth R; Shelby, Bryan D; Angelova, Magdalena et al. (2013) Kaposi sarcoma-associated herpesvirus g protein-coupled receptor enhances endothelial cell survival in part by upregulation of bcl-2. Ochsner J 13:66-75
Jenning, Scott; Pham, Tri; Ireland, Shubha Kale et al. (2013) Bit1 in anoikis resistance and tumor metastasis. Cancer Lett 333:147-51

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