This proposal will examine the effects of acute and chronic lead administration, as well as other metals, on a number of defined synaptic inputs, and transmitters to identified neurons in hippocampus, cerebral corte, cerebellum and caudate. The effect of fetal and neonatal lead exposure on maturation of these same brain regions, studied using histological, histochemical, and electrophysiological techniques, will also be examined. All experiments will be carried out in brain pieces transplanted to the anterior chamber of the eye, permitting an isolated and selective activation of the various inputs to mature brain as well as an analysis of intrinsic vs. extrinsic or indirect factors in any lead-induced developmental abnormalities. Catecholamine-containing brainstem nuclei will be sequentially grafted to the anterior chamber before and after hippocampus, cerebellum, cerebral cortex or caudate. Isolated brain circuits upon which to test the effects of lead can be produced using these double transplants.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Scientist Award (K05)
Project #
5K05MH000289-09
Application #
3075633
Study Section
Research Scientist Development Review Committee (MHK)
Project Start
1980-01-01
Project End
1989-12-31
Budget Start
1988-01-01
Budget End
1988-12-31
Support Year
9
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045
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Freedman, R; Stromberg, I; Seiger, A et al. (1992) Initial studies of embryonic transplants of human hippocampus and cerebral cortex derived from schizophrenic women. Biol Psychiatry 32:1148-63
Palmer, M R; Henschen, A; Trok, K et al. (1991) Functional innervation of spinal cord tissue by fetal neocortical grafts in oculo: an electrophysiological study. Exp Brain Res 87:96-107
Hoffer, B J; Olson, L; Palmer, M R (1987) Toxic effects of lead in the developing nervous system: in oculo experimental models. Environ Health Perspect 74:169-75
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