Abstract

of Work: Neutrophils in whole blood, buffy coats, and those isolated by PercollR gradients have been characterized with respect to Complement receptor type 3 (CR-3) expression. Basal as well as upregulated levels of CR-3 expression have been studied using a mouse monoclonal antibody to CR-3 and goat anti- mouse F(ab')2 fragments using an Ortho 50-H Flow Cytometer to selectively analyze the neutrophil population fluorescence histograms have been generated to discern levels of receptor expression. Preliminary studies have characterized maximal receptor expression. Upregulation due to incubation and isolation procedures have also been monitored. To date no significant difference has been detected in the ability of neutrophils from chemotactically depressed and non-depressed localized juvenile periodontitis patients to upregulate CR-3 compared to the normal population.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
5K16DE000158-03
Application #
3939916
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
State University of New York at Buffalo
Department
Type
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260

  Publications

Krebs, Linda J; Wang, Xiaopeng; Nagy, Atilla et al. (2002) Bombesin and epidermal growth factor potentiate the effect of cytotoxic LH-RH analog AN-152 in vitro. Int J Oncol 21:1325-9
Krebs, Linda J; Wang, Xiaopeng; Nagy, Attila et al. (2002) A conjugate of doxorubicin and an analog of Luteinizing Hormone-Releasing Hormone shows increased efficacy against oral and laryngeal cancers. Oral Oncol 38:657-663
Rogers, J D; Scannapieco, F A (2001) RegG, a CcpA homolog, participates in regulation of amylase-binding protein A gene (abpA) expression in Streptococcus gordonii. J Bacteriol 183:3521-5
Krebs, L J; Wang, X; Pudavar, H E et al. (2000) Regulation of targeted chemotherapy with cytotoxic lutenizing hormone-releasing hormone analogue by epidermal growth factor. Cancer Res 60:4194-9
Malek, R; Fisher, J G; Caleca, A et al. (1994) Inactivation of the Porphyromonas gingivalis fimA gene blocks periodontal damage in gnotobiotic rats. J Bacteriol 176:1052-9
Dolce, C; Anguita, J; Brinkley, L et al. (1994) Effects of sialoadenectomy and exogenous EGF on molar drift and orthodontic tooth movement in rats. Am J Physiol 266:E731-8
Stephan, E B; Dziak, R (1994) Effects of genistein, tyrphostin, and pertussis toxin on EGF-induced mitogenesis in primary culture and clonal osteoblastic cells. Calcif Tissue Int 54:409-13
Grossi, S G; Zambon, J J; Ho, A W et al. (1994) Assessment of risk for periodontal disease. I. Risk indicators for attachment loss. J Periodontol 65:260-7
Winston, J L; Chen, C K; Neiders, M E et al. (1993) Membrane protein expression by Actinobacillus actinomycetemcomitans in response to iron availability. J Dent Res 72:1366-73
Sharma, A; Sojar, H T; Lee, J Y et al. (1993) Expression of a functional Porphyromonas gingivalis fimbrillin polypeptide in Escherichia coli: purification, physicochemical and immunochemical characterization, and binding characteristics. Infect Immun 61:3570-3

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