Prostaglandin Receptor EP1 on Inflammatory Pathways. RUDE and B. Koller, Curriculum in Genetics and Molecular Biology University of North Carolina at Chapel Hill. Our goal is to produce transgenic mice deficient in the murine prostaglandin receptor EP1. We will then study the effects of the nullification of this receptor on inflammatory pathways. The murine EP1 receptor is comprised of 405 amino acids and has seven transmembrane spanning domains common to all G-proteincoupled receptors. Binding of prostaglandin-E2 to the membrane of cells possessing the EP1 receptor causes a rapid increase in intracellular calcium. EP1 is expressed abundantly in kidney and to a lesser amount in lung. To generate the transgenic mice, a murine genomic library was screened using a radiolabeled RT-PCR generated EP1 cDNA probe. DNA from the phage subclones was recovered and restricted with Not-1 to isolate the genomic insert from the rest of the phage DNA. The genomic insert was then cloned into a modified bluescript vector. This recombinant plasmid will be used to make a """"""""knock-out"""""""" construct which will be electroporated into ES cells. Cells in which gene targeting occurs by homologous recombination will be selected for and injected into blastocysts to form chimeric mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
3K16DE000165-10S1
Application #
3732413
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Moiseiwitsch, J R (2000) The role of serotonin and neurotransmitters during craniofacial development. Crit Rev Oral Biol Med 11:230-9
Moiseiwitsch, J R; Raymond, J R; Tamir, H et al. (1998) Regulation by serotonin of tooth-germ morphogenesis and gene expression in mouse mandibular explant cultures. Arch Oral Biol 43:789-800
Margolis, M J; Pajovic, S; Wong, E L et al. (1995) Interaction of the 72-kilodalton human cytomegalovirus IE1 gene product with E2F1 coincides with E2F-dependent activation of dihydrofolate reductase transcription. J Virol 69:7759-67
Venezie, R D; Toews, A D; Morell, P (1995) Macrophage recruitment in different models of nerve injury: lysozyme as a marker for active phagocytosis. J Neurosci Res 40:99-107
Venezie, R D; Vadiakas, G; Christensen, J R et al. (1994) Enamel pretreatment with sodium hypochlorite to enhance bonding in hypocalcified amelogenesis imperfecta: case report and SEM analysis. Pediatr Dent 16:433-6
Venezie, R D; Vann Jr, W F (1993) Pediatric dentists' participation in the North Carolina Medicaid program. Pediatr Dent 15:175-81
Washington, O R; Deslauriers, M; Stevens, D P et al. (1993) Generation and purification of recombinant fimbrillin from Porphyromonas (Bacteroides) gingivalis 381. Infect Immun 61:1040-7
Ignelzi Jr, M A; Padilla, S S; Warder, D E et al. (1992) Altered expression of pp60c-src induced by peripheral nerve injury. J Comp Neurol 315:171-7
Dmytryk, J J; Fox, S C; Moriarty, J D (1990) The effects of scaling titanium implant surfaces with metal and plastic instruments on cell attachment. J Periodontol 61:491-6
Rath, E M; Essick, G K (1990) Perioral somesthetic sensibility: do the skin of the lower face and the midface exhibit comparable sensitivity? J Oral Maxillofac Surg 48:1181-90

Showing the most recent 10 out of 12 publications