Abstract

I am continuing my thesis research using a murine plasma cell tumor model. Preliminary results show significant decreases in CD4+ T cells in both peripheral Iymph nodes (PLNs) and mesenteric Iymph nodes (MLNs) at the site of the intraperitoneal ascitic tumor. The percentage of B cells (B220+) expressing the maturation marker CD23 is unchanged in both PLNs and MLNs of tumor-bearing mice. I have extended my study to include lymphocytes from bone marrow, focusing on the developing B cell compartment. Identification of the various B cell populations will be done by flow cytometry using fluorescent-conjugated antibodies to B220, HSA, IgD, IgM, Pgp-1, and CD23 with rat IgG as negative control. Functional studies for immunoglobulin secretion will be done on cell culture supernatants by standard sandwich ELISA for mouse ID ano ILM.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
2K16DE000270-06
Application #
5210054
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Luger, Nancy M; Mach, David B; Sevcik, Molly A et al. (2005) Bone cancer pain: from model to mechanism to therapy. J Pain Symptom Manage 29:S32-46
Sevcik, Molly A; Luger, Nancy M; Mach, David B et al. (2004) Bone cancer pain: the effects of the bisphosphonate alendronate on pain, skeletal remodeling, tumor growth and tumor necrosis. Pain 111:169-80
Basi, David L; Ross, Karen F; Hodges, James S et al. (2003) The modulation of tissue factor by endothelial cells during heat shock. J Biol Chem 278:11065-71
Mach, D B; Rogers, S D; Sabino, M C et al. (2002) Origins of skeletal pain: sensory and sympathetic innervation of the mouse femur. Neuroscience 113:155-66
Sabino, Mary Ann C; Honore, Prisca; Rogers, Scott D et al. (2002) Tooth extraction-induced internalization of the substance P receptor in trigeminal nucleus and spinal cord neurons: imaging the neurochemistry of dental pain. Pain 95:175-86
Tran, S D; Rudney, J D; Sparks, B S et al. (2001) Persistent presence of Bacteroides forsythus as a risk factor for attachment loss in a population with low prevalence and severity of adult periodontitis. J Periodontol 72:1-10
Herzberg, M C (2001) Coagulation and thrombosis in cardiovascular disease: plausible contributions of infectious agents. Ann Periodontol 6:16-9
O'Brien, T P; Roszkowski, M T; Wolff, L F et al. (1996) Effect of a non-steroidal anti-inflammatory drug on tissue levels of immunoreactive prostaglandin E2, immunoreactive leukotriene, and pain after periodontal surgery. J Periodontol 67:1307-16
Reeh, E S; Ross, G K (1994) Tooth stiffness with composite veneers: a strain gauge and finite element evaluation. Dent Mater 10:247-52
Skopek, R J; Liljemark, W F (1994) The influence of saliva on interbacterial adherence. Oral Microbiol Immunol 9:19-24

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