Abstract

Tissue factor is produced and released by endothelial cells soon after injury and is important in the initiation of the coagulation cascade. It is unclear if elevated temperatures, associated with febrile illness, can affect tissue factor expression. In this study, we examined the expression of tissue factor by human endothelial vein cells (HUVECs) during heat shock. HUVECs were exposed to 44C heat shock for 0, 1, 2,4, or 8h. Tissue factor and heat shock protein 72-specific mRNAs were detected using ribonuclease protection assay at 1, 2,4, or 8h of heat shock. Heat shocked HUVECs express elevated levels protein 72 specific mRNA which peaked at 2 hours. Tissue factor specific mRNA was upregulated in unshocked HUVECs in response to LPS. However heat shocked HUVECs expressed reduced levels of TF mRNA in response to LPS during heat shock. These qualitative data suggest that transcriptional regulation in HUVECs differs for heat shock protein and tissue factor mRNA in response to heat shock. Regulated expression of tissue factor is affected by heat shock. Key words: wound healing, endothelial cell, differential display, infective endocarditis

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
5K16DE000270-11
Application #
6467397
Study Section
Project Start
2001-07-01
Project End
2002-06-30
Budget Start
Budget End
Support Year
11
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Minnesota Twin Cities
Department
Type
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455

  Publications

Luger, Nancy M; Mach, David B; Sevcik, Molly A et al. (2005) Bone cancer pain: from model to mechanism to therapy. J Pain Symptom Manage 29:S32-46
Sevcik, Molly A; Luger, Nancy M; Mach, David B et al. (2004) Bone cancer pain: the effects of the bisphosphonate alendronate on pain, skeletal remodeling, tumor growth and tumor necrosis. Pain 111:169-80
Basi, David L; Ross, Karen F; Hodges, James S et al. (2003) The modulation of tissue factor by endothelial cells during heat shock. J Biol Chem 278:11065-71
Mach, D B; Rogers, S D; Sabino, M C et al. (2002) Origins of skeletal pain: sensory and sympathetic innervation of the mouse femur. Neuroscience 113:155-66
Sabino, Mary Ann C; Honore, Prisca; Rogers, Scott D et al. (2002) Tooth extraction-induced internalization of the substance P receptor in trigeminal nucleus and spinal cord neurons: imaging the neurochemistry of dental pain. Pain 95:175-86
Tran, S D; Rudney, J D; Sparks, B S et al. (2001) Persistent presence of Bacteroides forsythus as a risk factor for attachment loss in a population with low prevalence and severity of adult periodontitis. J Periodontol 72:1-10
Herzberg, M C (2001) Coagulation and thrombosis in cardiovascular disease: plausible contributions of infectious agents. Ann Periodontol 6:16-9
O'Brien, T P; Roszkowski, M T; Wolff, L F et al. (1996) Effect of a non-steroidal anti-inflammatory drug on tissue levels of immunoreactive prostaglandin E2, immunoreactive leukotriene, and pain after periodontal surgery. J Periodontol 67:1307-16
Reeh, E S; Ross, G K (1994) Tooth stiffness with composite veneers: a strain gauge and finite element evaluation. Dent Mater 10:247-52
Skopek, R J; Liljemark, W F (1994) The influence of saliva on interbacterial adherence. Oral Microbiol Immunol 9:19-24

Showing the most recent 10 out of 11 publications