Mechanism of Bone Resorption

Alexander, Mary B.

Abstract

The Osteoclast is a multinucleated cell that actively resorbs bone. This bone resorptive process is an essential step in the sequential process of bone remodeling as well as in some disease processes. Stimulation of the osteoclast to resorb bone and inhibit the osteoblast from making bone probably involves the expression of an array of genes. The purpose of these studies is to attempt to elucidate the mechanism of bone resorption-related genes with emphasis on the characterization of these genes using the human osteoclastoma tumor system. Novel resorptive-related gene clones were obtained from an osteoclastoma cDNA library by differential screening using probes from the giant cell tumor and cultured stromal cells form the same tumor. In situ hybridization will be used to screen these genes for cell type specifity. Full length cDNA sequences will be respectively used for protein production and studies of regulation of resorption-related genes, in particular genes involved in osteoblast-osteoclast signaling. This approach has a high probability of uncovering osteoblast and osteoclast function related to the resorption process. Key Words: osteclast, osteclastoma, resorption related

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Unknown (K16)
• Project #: 5K16DE000275-04
• Application #: 3775636
• Support Year: 4
• Fiscal Year: 1993

Institution

• Name: Harvard University
• DUNS #: 082359691
• City: Boston
• State: MA
• Country: United States
• Zip Code: 02115

Publications

McDermott, Nancy E; Chuang, Sung-Kiang; Woo, Valerie V et al. (2006) Maxillary sinus augmentation as a risk factor for implant failure. Int J Oral Maxillofac Implants 21:366-74

Chuang, S K; Cai, T; Douglass, C W et al. (2005) Frailty approach for the analysis of clustered failure time observations in dental research. J Dent Res 84:54-8

Chuang, S-K; Hatch, J P; Rugh, J et al. (2005) Multi-center randomized clinical trials in oral and maxillofacial surgery: modeling of fixed and random effects. Int J Oral Maxillofac Surg 34:341-4

Treister, Nathaniel S; Woo, Sook-Bin; O'Holleran, Eileen W et al. (2005) Oral chronic graft-versus-host disease in pediatric patients after hematopoietic stem cell transplantation. Biol Blood Marrow Transplant 11:721-31

Woo, Valerie V; Chuang, Sung-Kiang; Daher, Shadi et al. (2004) Dentoalveolar reconstructive procedures as a risk factor for implant failure. J Oral Maxillofac Surg 62:773-80

Halpern, Leslie R; Carter, Jeffrey B; Chuang, Sung-Kiang et al. (2003) A comparison of 2 consultation and treatment strategies to manage impacted third molars. J Oral Maxillofac Surg 61:779-84

Basile, John R; Eichten, Alexandra; Zacny, Valerie et al. (2003) NF-kappaB-mediated induction of p21(Cip1/Waf1) by tumor necrosis factor alpha induces growth arrest and cytoprotection in normal human keratinocytes. Mol Cancer Res 1:262-70

McDermott, Nancy E; Chuang, Sung-Kiang; Woo, Valerie V et al. (2003) Complications of dental implants: identification, frequency, and associated risk factors. Int J Oral Maxillofac Implants 18:848-55

Chuang, S K; Tian, L; Wei, L J et al. (2002) Predicting dental implant survival by use of the marginal approach of the semi-parametric survival methods for clustered observations. J Dent Res 81:851-5

Chuang, S K; Wei, L J; Douglass, C W et al. (2002) Risk factors for dental implant failure: a strategy for the analysis of clustered failure-time observations. J Dent Res 81:572-7