. The innate-adaptive immune interface represents the site of action where components of both innate and adaptive immunity cross-engage each other to program the effector actions of the adaptive immune response. The actions of the innate-adaptive immune interface are considered essential for establishing protective immunity and immune memory against virus infection. Type III interferon (IFN-?) is a cytokine that functions at the innate-adaptive immune interface and is critical for mediating innate immune protection at mucosal barriers. IFN-? provides therapeutic benefit against Influenza A virus (IAV) infection, however how it operates within the innate-adaptive interface is not defined. We have utilized a murine model of IAV infection to study the contribution of IFN-? in regulation of immunity at the innate-adaptive interface during respiratory virus infection. Our studies show Ifnlr1-/- mice have blunted effector CD8+ T cell responses compared to WT mice and exhibit reduced survival upon heterosubtypic IAV re-challenge. Analysis of dendritic cells (DCs) reveals that IFN-? signaling directs CD103+ DC migration and function to develop optimal anti-viral CD8+ T cell responses. Further, preliminary bioinformatic analysis suggests IFN-? is essential for control of an Il10 immunoregulatory network in DCs during IAV infection. Our observations reveal that IFN-? bridges innate and adaptive immunity to direct DCs to program effective T cell immunity against IAV. We hypothesize IFN-? signaling in DC regulates an IL-10 program critical for development of effective T cell memory for lasting immunity against IAV. Thus, the studies in this proposal aim to: 1) determine the contribution of IFN-? to generation of memory CD4+ and CD8+ T cell responses and 2) elucidate IFN-? regulation of IL-10 in programming DC functions. Results from these studies will define the role of IFN-? at the innate-adaptive immune interface in programming effective immunity against IAV infection and inform IFN-?-based vaccine and immune therapy strategies to prevent and limit infection. !

Public Health Relevance

. IFN-? contributes to immunity against influenza A virus (IAV) infection at the interface of innate and adaptive immunity, but how IFN-? regulates the protective capacity of adaptive immune responses is not known. Our work has identified a critical role for IFN-? in regulating DC responses to activate CD8+ T cells during IAV infection. The work proposed here will define the contribution of IFN-? to the development of memory CD4+ and CD8+ T cell responses and define the mechanisms by which IFN-? programs DCs, revealing the full potential of IFN-? as a therapeutic against IAV. !

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Career Transition Award (K22)
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Allergy, Immunology, and Transplantation Research Committee (AITC)
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Gondre-Lewis, Timothy A
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Ohio State University
Schools of Medicine
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