This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. AIDS related: Novel therapies based on the adoptive transfer of unmodified or genetically modified T cells are being developed by several investigators to restore or improve host immune responses to viral infection and malignancy. The growth of T cells in vitro to numbers sufficient to modulate host immunity requires the addition of large numbers of feeder cells to the cultures to provide nutrients and growth factors. Peripheral blood mononuclear cells (PBMC) harvested from normal donors by leukapheresis and then subjected to gamma irradiation to prevent cell growth have been identified as an excellent source of feeder cells and are needed for the development of specific adoptive immunotherapy. This protocol enrolls normal volunteer donors for leukapheresis to obtain PBMC for use in developing immune-based therapies.
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