Abstract

The goal of this protocol is to determine whether staphylococcal toxins (superantigens) contribute to the T cell activation found in atopic dermatitis (AD) and study the role of S. Aureus infection in the pathogenesis of this common skin disease.
Specific Aim One will determine whether AD is associated with a selective expansion of T cells in their skin lesion.
Specific Aim Two determines whether the selective stimulation of T cells in AD is clonotypic or diverse by cloning and sequencing the individual T cell receptor (TCR) transcripts amplified from AD skin lesions. The final specific aim investigates whether S. Aureus growing on the skin of patients with an exacerbation of AD due to infection, produce toxins known to act as bacterial superantigens E.G. Staphylococcal enterotoxins (SES) and correlate these findings with the TCR VB gene usage of the t cell infiltrate from skin biopsies of the culture site. In addition, nonlesional skin of patients with AD and normal controls will be patch tested with staphylococcal exotoxins to determine they can induce eczematoid skin lesions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
General Clinical Research Centers Program (M01)
Project #
5M01RR000051-35
Application #
5218157
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
35
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

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