This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this pilot study is to develop an accurate and reproducible determination of GFR for children with chronic kidney disease. We wish to develop the pilot study at JHU, because we are one of two NIH-funded Clinical Coordinating Centers for the prospective study of Kidney Disease in Children, PI Susan Furth,MD, Ph.D. of Johns Hopkins University School of Medicine (NIH 1 U01 DK 066174-01). This pilot study of 15 patients is designed to test whether a 2-point plasma disappearance of iohexol (readily available as OmnipaqueR) estimates kidney glomerular filtration rate (GFR) as well as a 10-point plasma disappearance of iohexol. Iohexol levels in plasma and urine will be determined by HPLC, under the direction of Tai Kwong, Ph.D. in the Central Laboratory at University of Rochester. The 2-point plasma disappearance of iohexol will then be derived from the original 10-point curve, using the 1-compartment approximation of Brochner-Mortenson 1. These paired comparisons will be analyzed by regression using the difference (10-point - 2-point) as the outcome and the average of the 10- and 2-point compulations as the independent variable, and modified Bland-Altman 2 graphical representations. Statistical assistance will be done in close collaboration with Dr. Alvaro Mu oz and staff of the Data Coordinating Center for the NIH funded Chronic Kidney Disease in Children s
Showing the most recent 10 out of 1014 publications