This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: Many metabolic studies have relied upon retrieving multiple muscle samples from a common site. Recent studies have suggested that obtaining multiple samples from a common site may not be reliable because of potential inflammation from the prior biopsy. Hypothesis: Muscle biopsies taken from a common site will not differ significantly from muscle biopsies taken from different sites and different legs.
Specific Aims and Procedures (summary): Six muscle samples total will be taken from 4 sites total on both legs. We will compare measures from single-biopsy sites with measures from multiple-biopsy sites Experimental Design (summary): We will study 25 subjects both male and female within the age range of 18-40 years. We will infuse stable isotopes of amino acids for 10 hours (0600-1600) to measure the uptake of the isotope into the target blood and muscle samples. Blood samples will be taken at specific time points to assure that adequate levels of isotope are present. Muscle samples will be taken at 3hrs, 6hrs and 9hrs after beginning the infusion of isotope. Subjects will arrive shortly before study begins and will be discharged shortly after the final biopsy is obtained. Significance (summary): A scientifically valid method for acquiring multiple muscle samples while minimizing risks to subjects will serve to ensure data reliability and enhance subject safety.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
General Clinical Research Centers Program (M01)
Project #
5M01RR000073-43
Application #
7378747
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Project Start
2006-04-01
Project End
2007-03-31
Budget Start
2006-04-01
Budget End
2007-03-31
Support Year
43
Fiscal Year
2006
Total Cost
$5,350
Indirect Cost
Name
University of Texas Medical Br Galveston
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
800771149
City
Galveston
State
TX
Country
United States
Zip Code
77555
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