This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. HYPOTHESIS: The primary objective of this project is to characterize the circadian (daily) patterns of blood components in human subjects, with the ultimate goal of identifying factors that are involved in the control of daily patterns of tissue activity. We will also compare the profiles in subjects that are allowed to sleep vs. not allowed to sleep; this comparison may allow the identification of novel factors that are involved in regulation of sleep. This is a Pilot Study with the intention of collecting preliminary data for a full R01 proposal to the NIH.
SPECIFIC AIMS : To accomplish this goal, we will collect blood samples at different phases of the day and night in subjects over 36 hours. The experiment will be conducted as a crossover design, where each of three subjects will be exposed to two protocols: in Protocol 1, the subjects will be allowed to sleep at their normal sleeping time, and in Protocol 2, the subjects will stay awake during the 36 h collection time course. There will be a pause of several days between the protocols. The order of protocol administration (i.e., Protocol 1 first vs. Protocol 2 first) will be randomized among the subjects. Red blood cells will be removed from the samples and the remaining serum will be sent to a mass spectrometry facility for analysis of the components (and relative abundances) of 60-80 hormones/peptides/proteins in the plasma. Particular attention will be paid to EGF, FGF, melatonin, and cortisol.
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