This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human serum is the most commonly collected clinical specimen. In addition to the abundant proteins such as albumin, human serum also contains tissue proteins that are released into the bloodstream as a result of cellular changes such as damage or death. Since most of human biology is probably more complex than what a single protein concentration can reflect, analysis of human serum has been shifting to the study of multi-protein panels. Analysis of serum multi-protein panels has been successfully used to establish distinct serum protein profiles for ovarian, breast, and prostate cancer. In addition to disease, many factors such as genetics (race), age, gender and drugs influence the concentration of proteins in the serum. Collecting well-characterized samples for each research question is time consuming and expensive. Therefore the overall goal of this project is to establish a serum bank of normal subjects and subjects with various diseases that can be used to as control standards during testing of sera of patients with various diseases and disorders. To verify the suitability of this approach, we will use these samples to establish the distinctive serum protein profiles of patients with osteosarcoma, using the osteosarcoma serum samples that are currently available for analysis from the CHTN bank. The goal of this proposal is to establish the feasibility to collect samples from multiple sites using different target populations. The expected outcome of the overall project is a serum bank of robust, well-characterized and tested samples from the pediatric population that can be used as controls in retrospective clinical trials for multiple diseases. The expected outcome of this proposal is preliminary data on the feasibility of the recruitment and collection strategies.
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