This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to isolate the pituitary response to GnRH as part of our studies to determine the effects of aging and estrogen feedback on the hypothalamic-pituitary components of the reproductive axis in women. The protocol involves two 28-hr inpatient visits to the GCRC with baseline sampling and sc administration of 150 mcg/kg of the NAL-GLU GnRH antagonist (IND# 30,016) followed by administration of 25, 75, 250 and 750 ng/kg of GnRH (IND# 13,353) administered in random order with the exception that the highest dose is always administered as the last dose. Subjects are studied at baseline and after 28 days of transdermal estrogen administration. The order of testing is not randomized due to the potential carry-over effect of estradiol. Women who have not had a hysterectomy will receive 5 mg of medroxyprogesterone acetate for 10 days at the time of discharge from their second admission. Follow up with be done 30 days after their final admission.
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