This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to determine the importance of insulin in maintaining protein synthesis in children with Type 1 Diabetes. It is the hypothesis of the study that the requirement of insulin for protein metabolism is different from the insulin requirement for carbohydrate metabolism and that maintaining children in glycemic control may not provide sufficient insulin for anabolic responses of protein metabolism associated with growth. We will address this hypothesis by examining the anabolic effect of insulin in pubertal children with type 1 diabetes. Whole-body protein metabolism will be determined in the post absorptive state without insulin and then with the provision of insulin, amino acids, and glucose. The acute effect of insulin on whole-body protein metabolism will be assessed from the kinetics of L-[1-13C] leucine. By determining the protein balance in the presence of insulin at levels comparable to those achieved during normal feeding, we will be able to ascertain whether the children are sufficient protein balance to maintain growth.
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