This project is aimed at using the SV40 large T antigen to elucidate the mechanism(s) of cellular senescence in WI-38 cells. We intend to use the ability of SV40 large T antigen to induce DNA synthesis in senescent WI- 38 cells to study 3 aspects of cellular senescence: 1) the expression of the PCNA gene; 2) the roles of B-myb, IGF-1 receptor; and 3) the immortalization of HDF by a ts mutuant of SV40 large T antigen.
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| Gerhard, Glenn S; Benko, Floyd A; Allen, R G et al. (2002) Mitochondrial DNA mutation analysis in human skin fibroblasts from fetal, young, and old donors. Mech Ageing Dev 123:155-66 |
| Mawal-Dewan, Madhu; Lorenzini, Antonello; Frisoni, Lorenza et al. (2002) Regulation of collagenase expression during replicative senescence in human fibroblasts by Akt-forkhead signaling. J Biol Chem 277:7857-64 |
| Macera-Bloch, Lisa; Houghton, JeanMarie; Lenahan, Melanie et al. (2002) Termination of lifespan of SV40-transformed human fibroblasts in crisis is due to apoptosis. J Cell Physiol 190:332-44 |
| Lorenzini, Antonello; Tresini, Maria; Mawal-Dewan, Madhu et al. (2002) Role of the Raf/MEK/ERK and the PI3K/Akt(PKB) pathways in fibroblast senescence. Exp Gerontol 37:1149-56 |
| Tevethia, M J; Ozer, H L (2001) SV40-mediated immortalization. Methods Mol Biol 165:185-99 |
| Torres, C; Li, M; Walter, R et al. (2001) T-kininogen inhibits fibroblast proliferation in the G(1) phase of the cell cycle. Exp Cell Res 269:171-9 |
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