Abstract

Genetic concepts and methodologies serve as the integrating themes for an analysis of several basic phenomena of importance to gerontology. I. A) An already well developed program of research on the in vitro clonal senescence of normal mammalian somatic cells will couple somatic cell genetic investigations with cell kinetic and biochemical analyses to determine the mechanisms limiting the replicative potentials of cultured normal cells and the contrasts in cell cycle regulation between normal cells, cells from patients with Werner syndrome, and neoplastic cells. B) The in vivo clonal senescence of normal hematopoietic cells will also be investigated. II. A newly emerging program of research involving the use of cultured mouse teratocarcinoma cells for the genetic analysis of in vivo aging will be expanded and will include efforts to carry out several types of interspecific gene transfers. III. A newly proposed program of comparative biogerontology, involving Mus musculus, Peromyscus leucopus and Notomys alexis will be coupled with I and II above and will be utilized for tests of the intrinsic mutagenesis and free-radical theories of aging.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG001751-15
Application #
3090592
Study Section
Biological and Clinical Aging Review Committee (BCA)
Project Start
1979-09-29
Project End
1996-07-31
Budget Start
1993-08-15
Budget End
1994-07-31
Support Year
15
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Basisty, Nathan B; Liu, Yuxin; Reynolds, Jason et al. (2018) Stable Isotope Labeling Reveals Novel Insights Into Ubiquitin-Mediated Protein Aggregation With Age, Calorie Restriction, and Rapamycin Treatment. J Gerontol A Biol Sci Med Sci 73:561-570
Kramer, Philip A; Duan, Jicheng; Gaffrey, Matthew J et al. (2018) Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle. Redox Biol 17:367-376
Zhang, Huiliang; Gong, Guohua; Wang, Pei et al. (2018) Heart specific knockout of Ndufs4 ameliorates ischemia reperfusion injury. J Mol Cell Cardiol 123:38-45
Ge, Xuan; Ciol, Marcia A; Pettan-Brewer, Christina et al. (2017) Self-motivated and stress-response performance assays in mice are age-dependent. Exp Gerontol 91:1-4
Sweetwyne, Mariya T; Pippin, Jeffrey W; Eng, Diana G et al. (2017) The mitochondrial-targeted peptide, SS-31, improves glomerular architecture in mice of advanced age. Kidney Int 91:1126-1145
Liu, Sophia Z; Marcinek, David J (2017) Skeletal muscle bioenergetics in aging and heart failure. Heart Fail Rev 22:167-178
Chiao, Ying Ann; Kolwicz, Stephen C; Basisty, Nathan et al. (2016) Rapamycin transiently induces mitochondrial remodeling to reprogram energy metabolism in old hearts. Aging (Albany NY) 8:314-27
Campbell, Matthew D; Marcinek, David J (2016) Evaluation of in vivo mitochondrial bioenergetics in skeletal muscle using NMR and optical methods. Biochim Biophys Acta 1862:716-724
Basisty, Nathan; Dai, Dao-Fu; Gagnidze, Arni et al. (2016) Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy? Aging Cell 15:634-45
Treuting, P M; Snyder, J M; Ikeno, Y et al. (2016) The Vital Role of Pathology in Improving Reproducibility and Translational Relevance of Aging Studies in Rodents. Vet Pathol 53:244-9

Showing the most recent 10 out of 285 publications