The Specific Aims of this Core are: 1) To make readily available flow and image cytometry and cell sorting instrumentation and techniques to Program Project research. 2) To assist investigators in finding appropriate ways to apply cytometric techniques to their research objectives. The Core operates 4 cytometers, providing instrumentation that best matches the needed application. These are: an ELITE cell sorter; an ORTHO 50/2150 cell sorter, an ICP-22 non-sorting cytometer and an ACAS (Adherent Cell Analysis and Sorting) 570 imaging and confocal cytometer, In addition, software in support of Core cytometry services is developed and applied. The Core will provide the Program Project both scientific expertise and access to advanced instrumentation and software. Cytometry and cell sorting will be essential parts of each of the Projects, providing both preparative (cell purification by sorting) and analytical services. Types of Cytometric analyses and sorting that will be performed include: 1) Sorting lymphocyte subsets; 2) Assays of cell proliferation and Cell Cycle; 3) Analysis of Heterokaryon and Cytoplast cell fusion products; 4) Alkaline unwinding DNA damage and repair assays. 5) Measurement of mitochondrial membrane potential and cellular redox status; 6) Measurement of Apoptosis; 7) Quantitating Intracellular Calcium and Glutathione or pH; 8) Sorting of transfected cells and characterization of transgenic mouse tissues.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
2P01AG001751-18
Application #
5204347
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
1996
Total Cost
Indirect Cost
Basisty, Nathan B; Liu, Yuxin; Reynolds, Jason et al. (2018) Stable Isotope Labeling Reveals Novel Insights Into Ubiquitin-Mediated Protein Aggregation With Age, Calorie Restriction, and Rapamycin Treatment. J Gerontol A Biol Sci Med Sci 73:561-570
Kramer, Philip A; Duan, Jicheng; Gaffrey, Matthew J et al. (2018) Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle. Redox Biol 17:367-376
Zhang, Huiliang; Gong, Guohua; Wang, Pei et al. (2018) Heart specific knockout of Ndufs4 ameliorates ischemia reperfusion injury. J Mol Cell Cardiol 123:38-45
Ge, Xuan; Ciol, Marcia A; Pettan-Brewer, Christina et al. (2017) Self-motivated and stress-response performance assays in mice are age-dependent. Exp Gerontol 91:1-4
Sweetwyne, Mariya T; Pippin, Jeffrey W; Eng, Diana G et al. (2017) The mitochondrial-targeted peptide, SS-31, improves glomerular architecture in mice of advanced age. Kidney Int 91:1126-1145
Liu, Sophia Z; Marcinek, David J (2017) Skeletal muscle bioenergetics in aging and heart failure. Heart Fail Rev 22:167-178
Basisty, Nathan; Dai, Dao-Fu; Gagnidze, Arni et al. (2016) Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy? Aging Cell 15:634-45
Treuting, P M; Snyder, J M; Ikeno, Y et al. (2016) The Vital Role of Pathology in Improving Reproducibility and Translational Relevance of Aging Studies in Rodents. Vet Pathol 53:244-9
Ahn, Eun Hyun; Lee, Seung Hyuk; Kim, Joon Yup et al. (2016) Decreased Mitochondrial Mutagenesis during Transformation of Human Breast Stem Cells into Tumorigenic Cells. Cancer Res 76:4569-78
Kruse, Shane E; Karunadharma, Pabalu P; Basisty, Nathan et al. (2016) Age modifies respiratory complex I and protein homeostasis in a muscle type-specific manner. Aging Cell 15:89-99

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