The Scientific Core exists to facilitate the scientific projects within the program. The services, materials and reagents provided by the Scientific Core are purified scrapie prions, PrPs antibodies, synthetic peptides and oligonucleotides, and an analytical service for peptides and proteins. This core will be directed by Dr. Michael Baldwin, an analytical chemist of considerable experience who directed a core service laboratory in the University of London for 14 years and who has devoted himself to research into prion chemistry and biochemistry for the last 4 years. the preparation of purified prions has been carried out in this laboratory for more that a decade. In recent years this program has been supervised by Ms. Hana Serban. She will oversee the work of 2 SRA II's who will carry out the large-scale purifications required for projects II and III and for antibody production. PrP antibodies, crucial to the success of most of these projects, will be prepared and supplied by another SRAII. A further SRA II/III will synthesize peptides and oligonucleotides; the appointment of a new person to this post is essential to cope with the high volume of work that will be created by this program. Peptides are required for antibody production and for a variety of other purposes including binding studies, structural analysis and active site/infectivity for the production of recombinant Fab's and in project IV for construction of cosmids for transgenic mouse production and for screening these mice. Peptide and protein analysis will also be carried out by a further SRAII. All of these activities are currently being carried out very successfully in this laboratory and there are highly experienced personnel already in post.
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| Woerman, Amanda L; Watts, Joel C; Aoyagi, Atsushi et al. (2018) ?-Synuclein: Multiple System Atrophy Prions. Cold Spring Harb Perspect Med 8: |
| Woerman, Amanda L; Kazmi, Sabeen A; Patel, Smita et al. (2018) Familial Parkinson's point mutation abolishes multiple system atrophy prion replication. Proc Natl Acad Sci U S A 115:409-414 |
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| Condello, Carlo; Lemmin, Thomas; Stöhr, Jan et al. (2018) Structural heterogeneity and intersubject variability of A? in familial and sporadic Alzheimer's disease. Proc Natl Acad Sci U S A 115:E782-E791 |
| Woerman, Amanda L; Kazmi, Sabeen A; Patel, Smita et al. (2018) MSA prions exhibit remarkable stability and resistance to inactivation. Acta Neuropathol 135:49-63 |
| Yang, Bing; Wu, Haifan; Schnier, Paul D et al. (2018) Proximity-enhanced SuFEx chemical cross-linker for specific and multitargeting cross-linking mass spectrometry. Proc Natl Acad Sci U S A 115:11162-11167 |
| Wang, Tuo; Jo, Hyunil; DeGrado, William F et al. (2017) Water Distribution, Dynamics, and Interactions with Alzheimer's ?-Amyloid Fibrils Investigated by Solid-State NMR. J Am Chem Soc 139:6242-6252 |
| Giles, Kurt; Woerman, Amanda L; Berry, David B et al. (2017) Bioassays and Inactivation of Prions. Cold Spring Harb Perspect Biol 9: |
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