This project involves the development and validation of radio-immunoassays for the measurement of """"""""bone-specific"""""""" proteins in serum and urine. These will be used to assess bone formation and resorption in age-related bone loss and metabolic bone diseases. At first, the focus will be on bone Gla protein, osteonectin; then, on other noncollagenous bone-specific proteins, as these are isolated in Project 0003 and antibodies are developed. Assay of skeletal alkaline phosphatase which is near clinical usefulness will also be utilized in this project. A library of monoclonal and polyclonal antibodies to the proteins isolated on Project 0003 will be developed. Normal values will be established in healthy individuals of both sexes from ages 20-90 years. Criteria for exclusion of patients with bone disease are outlined. Studies will then be performed in patients with osteoporosis, hyperparathyroidism, hyperthyroidism, Paget's disease, steriod-induced osteoporosis, and other metabolic bone diseases, as well as patients with osteogenic sarcoma, and carcinoma of the prostate. The relationship to vitamin D metabolism will be evaluated by comparing serum levels in patients with vitamin D deficiency and subjects with hypervitaminosis D.
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| Khosla, Sundeep; Farr, Joshua N; Kirkland, James L (2018) Inhibiting Cellular Senescence: A New Therapeutic Paradigm for Age-Related Osteoporosis. J Clin Endocrinol Metab 103:1282-1290 |
| Rocca, Walter A (2018) The future burden of Parkinson's disease. Mov Disord 33:8-9 |
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| Laughlin-Tommaso, Shannon K; Khan, Zaraq; Weaver, Amy L et al. (2018) Cardiovascular and metabolic morbidity after hysterectomy with ovarian conservation: a cohort study. Menopause 25:483-492 |
| Farr, Joshua N; Weivoda, Megan M; Nicks, Kristy M et al. (2018) Osteoprotection Through the Deletion of the Transcription Factor Ror? in Mice. J Bone Miner Res 33:720-731 |
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| Kattah, Andrea G; Smith, Carin Y; Gazzuola Rocca, Liliana et al. (2018) CKD in Patients with Bilateral Oophorectomy. Clin J Am Soc Nephrol 13:1649-1658 |
| Khosla, Sundeep; Monroe, David G (2018) Regulation of Bone Metabolism by Sex Steroids. Cold Spring Harb Perspect Med 8: |
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