Abstract

This core will provide basic nucleic acid and recombinant DNA procedures for the program project. It will provide equipment and personnel specifically for the growth and isolation of plasmids or bacteriophage containing cloned DNA and for DNA sequencing. All of the projects will make use of this core. A major rationale for the establishment of this core was that the large volume of plasmid preparation envisioned by Project 3 required the purchase of an additional ultracentrifuge. By placing this item in a core facility along with other items needed for plasmid preparation, other investigators will have the benefit of the equipment and personnel. This core will also provide a DNA sequencing service for the program project as a whole. By providing specialized equipment, techniques and personnel, this core will increase the efficiency of the program project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
1P01AG007123-01
Application #
3821932
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Duttaroy, A; Qian, J F; Smith, J S et al. (1997) Up-regulated P21CIP1 expression is part of the regulation quantitatively controlling serum deprivation-induced apoptosis. J Cell Biochem 64:434-46
Smith, J R; Nakanishi, M; Robetorye, R S et al. (1996) Studies demonstrating the complexity of regulation and action of the growth inhibitory gene SDI1. Exp Gerontol 31:327-35
Aggarwal, B B; Totpal, K; LaPushin, R et al. (1995) Diminished responsiveness of senescent normal human fibroblasts to TNF-dependent proliferation and interleukin production is not due to its effect on the receptors or on the activation of a nuclear factor NF-kappa B. Exp Cell Res 218:381-8
Yang, L; Didenko, V V; Noda, A et al. (1995) Increased expression of p21Sdi1 in adrenocortical cells when they are placed in culture. Exp Cell Res 221:126-31
Kaul, S C; Wadhwa, R; Matsuda, Y et al. (1995) Mouse and human chromosomal assignments of mortalin, a novel member of the murine hsp70 family of proteins. FEBS Lett 361:269-72
Wadhwa, R; Pereira-Smith, O M; Reddel, R R et al. (1995) Correlation between complementation group for immortality and the cellular distribution of mortalin. Exp Cell Res 216:101-6
Nakanishi, M; Robetorye, R S; Pereira-Smith, O M et al. (1995) The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition. J Biol Chem 270:17060-3
Hensler, P J; Pereira-Smith, O M (1995) Human replicative senescence. A molecular study. Am J Pathol 147:1-8
Khaoustov, V I; Ozer, A; Smith, J R et al. (1995) Induction of senescent cell-derived inhibitor of DNA synthesis gene, SDI1, in hepatoblastoma (HepG2) cells arrested in the G2-phase of the cell cycle by 9-nitrocamptothecin. Lab Invest 73:118-27
Nakanishi, M; Robetorye, R S; Adami, G R et al. (1995) Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1. EMBO J 14:555-63

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