Abstract

The tissue core will process human articular cartilage and isolate chondrocytes. Cells will be stimulated in high density primary culture or in alginate beads and provided to the projects for experiments. Protein and mRNA extracts will be prepared from these specimens. The studies proposed in all research components depend on these samples and this core is thus essential to the success of the program. The specific functions of the core are: 1) Isolation of chondrocytes and perichondrial cells. 2) Expansion of a cell bank with chondrocytes from different age groups. 3) Chondrocyte cultures in monolyers and alginate. 4) Preparation of cells, conditioned media, protein and nucleic acid extracts from different culture systems and stimulation conditions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
2P01AG007996-08
Application #
6267391
Study Section
Project Start
1998-06-01
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
8
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Alvarez-Garcia, Oscar; Matsuzaki, Tokio; Olmer, Merissa et al. (2018) FOXO are required for intervertebral disk homeostasis during aging and their deficiency promotes disk degeneration. Aging Cell 17:e12800
Miyaki, Shigeru; Lotz, Martin K (2018) Extracellular vesicles in cartilage homeostasis and osteoarthritis. Curr Opin Rheumatol 30:129-135
Kalyanaraman, Hema; Schwaerzer, Gerburg; Ramdani, Ghania et al. (2018) Protein Kinase G Activation Reverses Oxidative Stress and Restores Osteoblast Function and Bone Formation in Male Mice With Type 1 Diabetes. Diabetes 67:607-623
Lee, Kwang Il; Olmer, Merissa; Baek, Jihye et al. (2018) Platelet-derived growth factor-coated decellularized meniscus scaffold for integrative healing of meniscus tears. Acta Biomater 76:126-134
Su, Alvin W; Chen, Yunchan; Wailes, Dustin H et al. (2018) Impact insertion of osteochondral grafts: Interference fit and central graft reduction affect biomechanics and cartilage damage. J Orthop Res 36:377-386
Chen, Liang-Yu; Lotz, Martin; Terkeltaub, Robert et al. (2018) Modulation of matrix metabolism by ATP-citrate lyase in articular chondrocytes. J Biol Chem 293:12259-12270
Matsuzaki, Tokio; Alvarez-Garcia, Oscar; Mokuda, Sho et al. (2018) FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis. Sci Transl Med 10:
Su, Alvin W; Chen, Yunchan; Dong, Yao et al. (2018) Biomechanics of osteochondral impact with cushioning and graft Insertion: Cartilage damage is correlated with delivered energy. J Biomech 73:127-136
Abhishek, Abhishek; Neogi, Tuhina; Choi, Hyon et al. (2018) Review: Unmet Needs and the Path Forward in Joint Disease Associated With Calcium Pyrophosphate Crystal Deposition. Arthritis Rheumatol 70:1182-1191
Fisch, K M; Gamini, R; Alvarez-Garcia, O et al. (2018) Identification of transcription factors responsible for dysregulated networks in human osteoarthritis cartilage by global gene expression analysis. Osteoarthritis Cartilage 26:1531-1538

Showing the most recent 10 out of 321 publications