The main objective of Core 9003 is to generated transgenic mice that are instrumental for Projects 4 and 5; to understand the role of neurotrophic factors, cholinergic and glutabminergic pathways in learning and memory, all of which are relevant to the biology of Alzheimer~s Disease. Furthermore these mice will facilitate Project 1 to develop a better gene transfer system in the brain. We will produce transgenic mice via microinjecting DNA into the pronucleus of one-cell embryos and gene targeting in mouse embryonic stem (ES) cells. We will be assisted by Core 9001 in the production of adenoviruses expressing the Cre recombinase that will be used to generate conditional mutant ES cell clones.
The specific aims are: 1. To generate transgenic mice by DNA microinjection. 2. To test reagents, maintain ES cell lines, and generate feeder cells for cultivating embryos and ES cells. 3. To generate null and conditional mutant ES cell lines by homologous recombination. 4. To generate chimeras for establishing germ- line mutant mice on an outbred or 129 inbred genetic background.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG010435-09
Application #
6098380
Study Section
Project Start
1999-03-01
Project End
2000-02-29
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
9
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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