The long-term goal of this research is to gain an understanding of the cellular and molecular events that lead to neuronal damage and death in Alzheimer's disease (AD). This project tests the hypothesis that beta- amyloid destabilizes cellular calcium homeostasis and thereby renders neurons more vulnerable to environmental insults. A hippocampal cell culture system will be used to examine the cellular and molecular mechanisms whereby beta-amyloid destabilizes neuronal calcium homeostasis, and potentiates excitatory amino acid neurotoxicity. Immunolocalization studies of AD brains are designed to determine whether the neuropathology of AD is consistent with the calcium destabilization hypothesis of beta- amyloid neurotoxicity.
The first aim will test the hypothesis that beta- amyloid affects specific cellular systems for calcium homeostasis (NMDA receptors, calcium channels Na+/Ca2+ exchanger, calcium binding protein and calcium ATPase).
The second aim i s to determine whether endogenous beta- amyloid contributes to selective neuronal vulnerability in cell culture.
The third aim i s to establish whether the cytoskeletal manifestations of neuronal degeneration induced by beta-amyloid resemble the neurofibrillary pathology of AD.
The fourth aim i s to determine the relationships of beta- amyloid, NMDA receptors, and calcium-regulating proteins in the histopathology of AD.
These aims will be accomplished using the following technologies: fluorescence ration imaging of intracellular calcium levels, and patch clamp studies of calcium currents in hippocampal neurons; immunocytochemistry to localize beta-amyloid and calcium-regulating proteins in cell cultures and in AD brains; confocal laser scanning microscopy; electron microscopy. Taken together, these studies will: (1) Provide insight into the cellular and molecular mechanisms whereby beta-amyloid destabilizes neuronal calcium homeostasis. (2) Tell us whether the cellular pathology of AD is consistent, at the molecular level, with the calcium-destabilization hypothesis. (3) Generate information that can be used to develop new approaches to preventing and treating the neuronal damage that is responsible for the progression of AD.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG010836-05S1
Application #
6234410
Study Section
Project Start
1997-09-01
Project End
1998-07-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
5
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Kentucky
Department
Type
DUNS #
832127323
City
Lexington
State
KY
Country
United States
Zip Code
40506
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Perluigi, Marzia; Di Domenico, Fabio; Buttterfield, D Allan (2014) Unraveling the complexity of neurodegeneration in brains of subjects with Down syndrome: insights from proteomics. Proteomics Clin Appl 8:73-85
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