The functions of the laboratory Core are: 1. to provide Individual Projects #1 and #2 with accurate and reproducible measurements of the levels of cortisol and melatonin in saliva samples. These non-invasive measurements will allow for th estimation of the onset of nocturnal melatonin secretion, a marker of circadian phase which is affected by the aging process, and the quiescent period of cortisol secretion, a marker of hypothalamo-pituitary-adrenal function which is affected by aging, stress and sleep loss. In contrast to plasma levels, saliva levels of cortisol reflect the concentrations of the free hormone, rather than the combination of free and bound hormone. 2. to provide Individual Project #2 with accurate and reproducible measurement of the levels of melatonin and thyroid-stimulating-hormone (TSH) in plasma samples collected in research subjects admitted to the University of Chicago Clinical Research Center. The Core laboratory will also prepare samples for the assay of blood levels of other hormones too be performed in the Laboratory of the Clinical Research Center and/or in the Diabetes Research and Training Center of th University of Chicago. 3. to provide Individual Project #3 with accurate and reproducible measurements of the levels of melatonin in blood samples collected from hamsters. 4. to provide Projects #1 and #2 with accurate and reproducible measurements of the plasma levels of catecholamines (epinephrine, norepinephrine and dopamine). The laboratory Core utilizes the existing facilities of the PI, Dr. Scherberg, and the Co-PI, Dr. Polonsky, at the University of Chicago. Both investigators have extensive experience in performing measurements for circadian rhythm studies. The availability of the major equipment needed to perform these measurements reduces the overall cost of these services to the program Project. The Core laboratory centralizes records on all hormonal measurements obtained in studies performed under the auspices of the Program Project.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
3P01AG011412-05S2
Application #
6218755
Study Section
Project Start
1999-09-30
Project End
1999-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Chicago
Department
Type
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637
Morselli, Lisa L; Gamazon, Eric R; Tasali, Esra et al. (2018) Shared Genetic Control of Brain Activity During Sleep and Insulin Secretion: A Laboratory-Based Family Study. Diabetes 67:155-164
Temple, Karla A; Leproult, Rachel; Morselli, Lisa et al. (2018) Sex Differences in the Impact of Obstructive Sleep Apnea on Glucose Metabolism. Front Endocrinol (Lausanne) 9:376
Morselli, Lisa L; Temple, Karla A; Leproult, Rachel et al. (2018) Determinants of Slow-Wave Activity in Overweight and Obese Adults: Roles of Sex, Obstructive Sleep Apnea and Testosterone Levels. Front Endocrinol (Lausanne) 9:377
Jiang, Peng; Turek, Fred W (2018) The endogenous circadian clock programs animals to eat at certain times of the 24-hour day: What if we ignore the clock? Physiol Behav 193:211-217
Hong, Hee-Kyung; Maury, Eleonore; Ramsey, Kathryn Moynihan et al. (2018) Requirement for NF-?B in maintenance of molecular and behavioral circadian rhythms in mice. Genes Dev 32:1367-1379
Guyon, Aurore; Morselli, Lisa L; Balbo, Marcella L et al. (2017) Effects of Insufficient Sleep on Pituitary-Adrenocortical Response to CRH Stimulation in Healthy Men. Sleep 40:
Baron, Kelly Glazer; Reid, Kathryn J; Malkani, Roneil G et al. (2017) Sleep Variability Among Older Adults With Insomnia: Associations With Sleep Quality and Cardiometabolic Disease Risk. Behav Sleep Med 15:144-157
Peek, Clara Bien; Levine, Daniel C; Cedernaes, Jonathan et al. (2017) Circadian Clock Interaction with HIF1? Mediates Oxygenic Metabolism and Anaerobic Glycolysis in Skeletal Muscle. Cell Metab 25:86-92
Mokhlesi, Babak; Grimaldi, Daniela; Beccuti, Guglielmo et al. (2017) Effect of one week of CPAP treatment of obstructive sleep apnoea on 24-hour profiles of glucose, insulin and counter-regulatory hormones in type 2 diabetes. Diabetes Obes Metab 19:452-456
Jiang, Peng; Turek, Fred W (2017) Timing of meals: when is as critical as what and how much. Am J Physiol Endocrinol Metab 312:E369-E380

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