Abstract

The Viral Vector Core Lab will assist the affiliated projects with large scale preparation and purification of viral vectors for the program project. This entails maintaining stocks of all necessary shuttle vectors and cell lines, performing the extensive serial passaging needed for amplification of viral stocks, and purifying these viral stocks by CsCl density gradient centrifugation. Finally, the core lab will assay the purified products to determine the titers of """"""""gutted"""""""" vector and helper virus,a s well as to detect any possible contamination with replication competent adenovirus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG015434-02
Application #
6098809
Study Section
Project Start
1999-05-01
Project End
2000-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Prins, Kurt W; Asp, Michelle L; Zhang, Huiliang et al. (2016) Microtubule-Mediated Misregulation of Junctophilin-2 Underlies T-Tubule Disruptions and Calcium Mishandling in mdx Mice. JACC Basic Transl Sci 1:122-130
Muir, Lindsey A; Nguyen, Quynh G; Hauschka, Stephen D et al. (2014) Engraftment potential of dermal fibroblasts following in vivo myogenic conversion in immunocompetent dystrophic skeletal muscle. Mol Ther Methods Clin Dev 1:14025
Nishimura, Mayuko; Kumsta, Caroline; Kaushik, Gaurav et al. (2014) A dual role for integrin-linked kinase and ?1-integrin in modulating cardiac aging. Aging Cell 13:431-40
Ramaswamy, Krishnan S; Palmer, Mark L; van der Meulen, Jack H et al. (2011) Lateral transmission of force is impaired in skeletal muscles of dystrophic mice and very old rats. J Physiol 589:1195-208
Claflin, Dennis R; Larkin, Lisa M; Cederna, Paul S et al. (2011) Effects of high- and low-velocity resistance training on the contractile properties of skeletal muscle fibers from young and older humans. J Appl Physiol 111:1021-30
Palmer, Mark L; Claflin, Dennis R; Faulkner, John A et al. (2011) Non-uniform distribution of strain during stretch of relaxed skeletal muscle fibers from rat soleus muscle. J Muscle Res Cell Motil 32:39-48
Gumerson, Jessica D; Kabaeva, Zhyldyz T; Davis, Carol S et al. (2010) Soleus muscle in glycosylation-deficient muscular dystrophy is protected from contraction-induced injury. Am J Physiol Cell Physiol 299:C1430-40
Kimura, En; Li, Sheng; Gregorevic, Paul et al. (2010) Dystrophin delivery to muscles of mdx mice using lentiviral vectors leads to myogenic progenitor targeting and stable gene expression. Mol Ther 18:206-13
Fink, Martin; Callol-Massot, Carles; Chu, Angela et al. (2009) A new method for detection and quantification of heartbeat parameters in Drosophila, zebrafish, and embryonic mouse hearts. Biotechniques 46:101-13
Wessells, Robert; Fitzgerald, Erin; Piazza, Nicole et al. (2009) d4eBP acts downstream of both dTOR and dFoxo to modulate cardiac functional aging in Drosophila. Aging Cell 8:542-52

Showing the most recent 10 out of 48 publications